摘要
目的获得有生物活性的抗乳腺癌扩增性抗原1氮端(amplifiedinbrestcancer1-Nterminal,AIB1-N)单克隆抗体。方法以谷胱甘肽转硫酶耦联的抗乳腺癌扩增性抗原1氮端蛋白(GST-AIB1-N)为免疫原,免疫BALB/c小鼠,采用杂交瘤技术制备抗AIB1-N单克隆抗体,用间接ELISA和Western-blot鉴定其亚类和抗原结合特异性。结果成功筛选出一株稳定分泌抗AIB1-N单克隆抗体的杂交瘤细胞株,其分泌抗体亚类为IgG1,经Western-blot鉴定该McAb特异性高,亲和力强。结论成功制备了抗AIB1-N单克隆抗体,为深入研究AIB1的表达及临床应用提供了有力的工具。
Objective To make and identify the monoclonal antibody against AIB1-N. Methods BALB/e mice were immunized with purified GST-AIB1-N protein, MeAb against AIB1-N was produced by hybridoma technique. ELISA and Western-blot were used to identify the immunoglobin subtype and specificity. Results A hybridoma cell was successfully produced to secrete the MeAb against AIB1-N, which was identified to belong in IgG1 subtype. By western-blot, the MeAb against AIB1 displayed strongly specificity and high affinity. Conclusion The MeAb against AIB1 protein may be a useful tool for studying the biological properties of AIB1 expression and the clinical laboratory detection.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2006年第2期278-280,297,共4页
Journal of Sichuan University(Medical Sciences)
基金
国家自然科学基金创新研究群体科学基金(批准号30221001)
四川省青年基金(编号04ZQ026-027及04SG022-009-2)资助
