摘要
目的比较胶体金免疫层析法(CGIA)和酶联免疫吸附法(ELISA)诊断肝吸虫病的特异性和准确性,探讨其在肝吸虫病诊断申的应用。方法采用CGIA和ELISA检测36例大便检出虫卵的肝吸虫病患者血清中的肝吸虫抗体。并设立健康人对照组和其它疾病患者作为阴性对照。结果 CGIA和ELISA对阳性标本检测的准确性分别为94.44％和97．22％；对阴性病例检测的特异性分别为98．06％和96．12％；对正常标本检测的特异性分别为97．78％和93．33％。结论 CGIA和ELISA对肝吸虫病患者血清抗体检测的准确性和特异性无显著性差异,两种方法的准确性和特异性均高,且操作简便,均可作为肝吸虫病的初诊及普查筛选方法。
Objective To evaluate the accuracy and specificity of colloidal gold immunochromatographic assay （CGIA） and enzyme- linked immunosorbent assay （ELISA） in the detection of clonorchiasis sinensis. Methods The antibody against Opisthorchis sinensis in the serum of clonorchiasis sinensis patients with worm eggs in the stool tested by microscopy was detected by CGIA and ELISA, respectively, Healthy subjects and patients with other diseases were taken as controls. Results The accurate rate were 94.44% and 97.22% in the detection of positive sample tested by CGIA and ELISA respectively. The specificity was 98. 06% and 96.12% in the detection of negative sample tested by CGIA and ELISA, and for normal sample the specificity was 97.78% and 93.33 %, respectively. Conclusion There was no difference in the accuracy and specificity of CGIA and ELISA in detecting the antibody against Opisthorchis sinensis , and both of the methods were sensitive, specific and simple in the diagnosis and screening of clonorchiasis sinensis.
出处
《中国热带医学》
CAS
2006年第3期394-395,共2页
China Tropical Medicine
关键词
胶体金免疫层析法
酶联免疫吸附法
肝吸虫病
Colloidal gold immunochromatographic assay
Enzyme linked immunosorbent assay
Clonorchiasis sinensis
