摘要
20年来国内外主要进行了快速生长转基因鱼、抗寒(耐寒)转基因鱼、抗病转基因鱼和其他抗性转基因鱼的研究,常用的鱼类基因转移技术有显微注射法、精子载体法、电穿孔法、基因枪法、胚胎干细胞载体、脂质体法和缺陷性病毒感染法等基因转移方法。转基因鱼的安全性和遗传稳定性有望通过进一步的科研攻关得到解决,转基因鱼的生产应用前景广阔。
Researches in transgenic fish in the past two decades home and abroad are mainly concentrated on several aspects including fast-growth transgenic fish, antifreeze/freeze-resistant transgenic fish and antidisease transgenic fish. Often used technologies for gene transfer in fish are sperm mediated gene transfer, electroporation, particle gun, embryonic stem cell insertion, liposomes, retrovirus infection,and so on. The safey and heredity stability of transgcnic fish could be lifted and finally solved through further research. Transgenic fish have broad and bright prospects in its future application.
出处
《广西科学》
CAS
2006年第1期76-80,共5页
Guangxi Sciences
基金
广西科学基金项目(桂科自0542035)资助
关键词
转基因鱼
显微注射
基因打靶
transgenic fish, microingection, gene targetting
