摘要
目的研究阿片受体拮抗剂纳洛酮对长期酒精摄入大鼠空间学习与记忆障碍的影响。方法SD大鼠随机分成三组:A正常对照组、B慢性酒精摄入组和C纳洛酮治疗组,给B、C组大鼠自由饮用由含6%逐渐递增至15%(v/v)的乙醇水溶液8周,建立慢性酒精中毒模型,8周后C组大鼠连续10d腹腔注射纳洛酮,其他组腹腔注射生理盐水,然后分组对动物进行Morris水迷宫训练。结果(1)在Morris水迷宫隐匿平台训练中,B、C组多个时段动物逃避潜伏期较A组长,差异有显著性(P<0.05或P<0.01)。纳洛酮治疗后C组[3d、4d、5d分别为:(36.50±4.19)s,(30.00±4.35)s,(18.59±3.64)s]较B组明显缩短,差异有显著性(P<0.05)。(2)探索实验中,B、C组在目标象限游泳时间都明显短于A组,差异有显著性(P<0.01或P<0.05),而C组明显长于B组,差异有显著性(P<0.05)。游泳路径的站台周边区域分布显示,三组采取了不同的搜索策略,A、C、B组游泳路径主要分布依次为站台周边区域Ⅰ、Ⅱ、Ⅲ,逐渐远离站台。结论长期酒精摄入后大鼠的空间记忆保持能力及空间定位的准确性都受损,而给予纳洛酮处理后能改善记忆保持能力。
Objective To study the effect of naloxone on spatial learning and memory of chronic ethanol intake（ CEI ） in rats. Methods Alcohol-induced learning and memory impairment model was established by 8 weeks continuous alcohol consumption. The rats with ethanol were randomly divided into two groups： group B treated by viz. naloxone injection（ i. p. ） for 10 days and group C non-treated model. Group C and group A （control） received the same volume saline injection. All rats were trained in Morris Water Maze orderly for 7 days. Spatial learning and memory abilities were evaluated with a series of tasks to find a hidden platform in Morris Water Maze. Results ①The escape latencies of the hidden platform in most trails in group B and C were significantly longer than group A （ P〈 0.01 or P 〈 0.05 ）. The escape latencies were significantly reduced in group C compared with group B （ P 〈 0.05）. ②In probe trails, the swimming time of target quadrant group B and C were significantly less group A （ P 〈 0.01 or P 〈 0.05 ）. The swimming time of group C was longer than group B （ P 〈 0.05 ）. The swimming paths in the different area away from platform indicated the different searching strategies among three groups. Conclusion Naloxone can ameliorate alcohol-induced learning and memory impairment in rats.
出处
《中国行为医学科学》
CAS
CSCD
2006年第3期213-215,共3页
Chinese Journal of Behavioral Medical Science
关键词
纳洛酮
学习记忆
酒精摄入
大鼠
Naloxone
Learning and memory
Ethanol intake
Rat
