摘要
目的建立大鼠脑多梗死性痴呆(MID)模型及观察指标,评价药物对该模型的作用。方法采用从颈外动脉逆行注入微血栓颗粒的方法,制备MID模型,通过对术后大鼠的苏醒时间测定、倾斜板实验、神经症状评分3个方面评价动物的一般状态;采用大鼠穿梭箱实验、水迷宫实验检测各组大鼠学习记忆能力;并测定脑中丙二醛(MDA)的含量及超氧化物歧化酶(SOD)的活性,考察MID大鼠的行为学异常以及其作用机制。结果MID大鼠与正常组、假手术组比较,苏醒时间明显延长,在倾斜板上停留的时间显著变短,神经症状评分有显著差异;穿梭箱实验中电击时间明显延长,水迷宫实验学习记忆潜伏期延长;同时脑组织中MDA含量明显升高,SOD活力显著降低。给予药物氢化麦角碱(商品名:喜得镇)治疗后症状有所改善。结论该造模方法能显著造成大鼠脑多梗死性痴呆的多项体征,符合临床病理过程,为进一步判定一些药物是否具有治疗MID的前景打下基础。
OBJECTIVE To establish a muhi-infarction dementia rat model and evaluate drugs effect.METHODS Homogenous microthrombus were injected into internal carotidartery through the external carotidartery of Wistar rats to get unilateral embelic cerebral multi-infarction. After the ischemic insult, revival time and slope board experiment were performed and stroke index was evaluated. The behavior of rats was measured by using shuttle box test and water maze test, the content of MDA and the activity of SOD in brain were measured. RESULTS Compared with the sham group, cerebral multi-infarction caused rats memorial dysfunction, the error numbers and stimulating time were increased in shuttle-box test. At the same time, the latency of water maze test was lengthened. The activity of SOD was reduced and the content of MDA was increased. After chug treatment, some symptoms were alleviated. CONCLUSION This model can be used to study vascular dementia because its pathomorphological manifestation is similar to the clinical one. This is the fundamental assurance for assessing the action of prescription and its mechanisms accurately. It is also the base for the research of anti-vascular dementia drugs.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2006年第5期347-350,共4页
Chinese Pharmaceutical Journal
关键词
微血栓
多梗死性痴呆
血管性痴呆
大鼠模型
microthrombus
multiple-infarction dementia
vascular dementia
rats model
