摘要
用高效液相色谱法测定大黄不同炮制品中大黄素的含量。采用Hypersil Gold C18柱（150mm×4．6mm，5μm），以甲醇-0．05％磷酸（85：15）为流动相，流速1．0mL／min，检测波长254nm。大黄素的线性检测范围为4．79—47．9μg／mL，线性良好，R^2=1．000；大黄素平均加样回收率为98．56％，RSD为1．30％（n=5）。生大黄及各炮制品中大黄素含量分别为：生大黄0．401％，熟大黄0．669％，酒大黄0．512％，醋大黄0．348％。试验得出如下结论：炮制使大黄中大黄素的含量产生明显变化。
An HPLC method to determine the emodin in different processed rhubarb was established with a Hypersil Gold C18 （ 150 mm × 4.6 mm, 5 μm） column. The mobile phase was composed of methanol and 0.05% H3PO4 solution （85： 15, V/V）, the flow rate was 1.0 mL/min, the detection wavelength was 254 nm, and the column temperature was room temperature. The calibration curve was linear in the concentration range of 4.79 - 47.9 μg/mL with correlation coefficient of 1. 000. The mean recovery of the method was 98.56%, RSD was 1.30% （ n = 5）. The concentrations of emodin in Radix et Rhizoma Rhea and its processed products are as follows, 0.401% in Radix et Rhizoma Rhei, 0.669% in ripe Radix et Rhizoma Rhei, 0.512% in winy Radix et Rhizoma Rhei, and 0.348% in acetic Radix et Rhizoma Rhei, respectively. The results showed that the content of emodin in Radix et Rhizoma Rhei was different from that in the corresponding processed drugs.
出处
《中国兽药杂志》
2006年第3期9-12,共4页
Chinese Journal of Veterinary Drug
