摘要
采用花粉管通道法，将青菜株系７６Ｆ５、１４号大白菜、紫阳等三个品种的总ＤＮＡ及质粒ｐＡｃｔｌ－ＤＤＮＡ导入青菜６０５品系，收获青菜种子．种子发芽后分别提取约两周苗龄的小苗总ＤＮＡ，并经ＥｃｏＲＩ酶切，以ｇｕｓ基因片段为探针做分子杂交．证实了外源ｇｕｓ基因已整合到青菜６０５品系中；田间观察还获得了一株与供体大白菜花形相似的变异株．
otal DNAs of Brassica chinensis L. strain 76F5, Brassica pekinensis L.,Brassica oleracea var rubra L. and plasmid pActl-D were introduced into Brassica chinensis L. variety 605 via pollen-tube pathway. Seeds from the foreign DNA-treated plants were harvested and allowed to germinate. Hybridization of the gus gene fragment probe to products of EcoR I-digested total DNAs from various plants were carried out and hybridization bands were detected in the total DNAs of progenies from foreign DNA-treated plants. A variant similar to the donor Brassica pekinensis L. was obtained among progenies.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1996年第2期127-131,共5页
Journal of Fudan University：Natural Science
基金
上海市科学技术发展基金
关键词
青菜
分子育种
花粉管通道
外源DNA导入
育种
cabbage
molecular breeding
pollen-tube pathway
introduction of foreign DNA
variant plant.
