摘要
根据绵羊β－乳球蛋白基因调控区ＤＮＡ序列设计了两个引物，以绵羊基因组ＤＮＡ为模板，用ＰＣＲ技术扩增，得约９００ｈＰ的ＤＮＡ片段插入到ｐＵＣ１８质粒的ＸｂａＩ／ＫｐｎＩ位点之间．ＰＣＲ产物的克隆经双酶切、ＰＣＲ检测和序列分析证明是绵羊β－乳球蛋白基因的调控序列．序列分析结果表明该克隆片段与绵羊β－乳球蛋白基因相应ＤＮＡ序列相比有很高的一致性．研究结果为指导异源基因的表达和进一步利用乳腺特异表达的转基因动物生产医用蛋白提供了有效的调控序列．
The regulatory sequence of the sheep β-lactoglobulin (BLG) gene was obtained by PCR amplification from the genomic DNA of the sheep. The fragment was inserted into the Kpnl/XbaIsite of pUC18. The results of retriction analysis and PCR and sequence analysis showed thatpBLG contains the regulatory region of the sheep BLG gene. The region shares 99% nucleotidesequence with the reported nucleotide sequence of the genomic ovine β-lactoglobulin. This expertment provided a regulatory sequence that it could direct the mammary-specific expression of thepharmaceutical protein gene in the transgenic animals.
出处
《内蒙古大学学报（自然科学版）》
CAS
CSCD
1996年第3期371-377,共7页
Journal of Inner Mongolia University：Natural Science Edition
基金
国家自然科学基金
关键词
绵羊
调控序列
PCR
β乳球蛋白基因
分离
无性系
sheep β-lactoglobulin (BLG) regulatory sequence polymerase chain reaction (PCR)
