摘要
以３０例肺癌、４０例胃癌及２２例肝癌石蜡包埋组织提取的ＤＮＡ为模板，经两轮ＰＣＲ扩增一限制性内切酶酶切，用限制性酶切片段长度多态性方法（ＲＦＬＰ）检测ｃ－Ｋｉ－ｒａｓ第１２密码子点突变。结果发现，肺癌组织中２０％（６／３０）有突变，其中４０％（４／１０）鳞癌和２０％（２／１０）腺癌有突变，小细胞肺癌（０／１０）无突变；４０例胃癌中仅１例（２．５％）有突变，２２例肝癌无１例突变。ＰＣＲ－ＲＦＬＰ是一种很敏感及快速检测基因突变的方法。
DNA extracted from paraffin-embedded tissues of 30 lung carcinomas,40 gastric carcinomas and 22 hepatocellular carcinomas was tested for the presence of c-Ki-ras codon 12point mutation by PCR-RFLP technique.The results showed that 20%(6/30)lung carcino-mas and 2.5%(1/40)gastric carcinomas had Ki-ras codon 12 point mutation,but non of the22 hepatocellular carcinomas displayed this point mutation,Among 6 cases of lung carcino-mas of mutation,4 were squamous carcinomas and 2 were adenocarcinomas.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1996年第2期95-98,共4页
Acta Academiae Medicinae Sinicae
基金
国家教委博士点基金
北京协和医院青年科研基金
关键词
肺肿瘤
胃肿瘤
肝脏肿瘤
RAS族
癌基因
点突变
carcinomas
ras gene
point mutation
polymerase chain reaction-restriction fragment length polymorphism
