摘要
以肝细胞场（ＨＣＣ）患者手术切除标本为研究材料，采用高效液相色谱荧光法检测组织中的黄曲霉毒素Ｂ１（ＡＦＢ１），嵌套式多聚酶链反应技术检测组织中的ＨＢＶＤＮＡ和ＨＣＶＲＮＡ。在６９．２％（１８／２６例）的ＨＣＣ患者中检出了３．６５－８９．０６ｎｇ不等的ＡＦＢ１／ｇ肝组织；ＨＢＶＤＮＡ和ＨＣＶＲＮＡ检出率分别为９０．５％（５７／６３例）和１２．７％（８／６３例）。其中，ＡＦＢ１和ＨＢＶＤＮＡ重叠检出率为６１．５％（１６／２６例），ＨＢＶＤＮＡ和ＨＣＶＲＮＡ重叠检出率为７．９％（５／６３例），４例ＨＣＣ患者重叠检出了ＡＦＢ１、ＨＢＶＤＮＡ和ＨＣＶＲＮＡ；同步检测对照组５例，无１例出现这种情况。结果表明，上述３种因素均与人类肝癌密切相关，并且可能存在协同致癌效应。
nalysis was made by using human liver tissues from hepatectomy specimens of patients with hepatocellular carcinoma (HCC) to detect aflatoxin B1 (AFB1) by high performance liq- uid chromatography (HPLC) , and HBV DNA by nested PCR technique ,and HCV RNA by reverse transcription nested PCR technique. AFB1 was detected in 18 of 26 (69. 2% ) patients with HCC,ranging from 3. 65-89. 06ng/g liver tissue. The positive rates of HBV DNA and HCV RNA were 90. 5%(57/63) and 12. 7%(8/63) respectively. Among these patients, AFB1 and HBV DNA were simultaneously detected in 61. 5 % ( 16/26 ) , and HCV RNA and HBV DNA in 7. 9% (5/63). Furthermore, AFB1,HBV DNA and HCV RNA were detected simultaneously in four patients with HCC. Whereas all of subjects of control group were negative for these three factors. These results suggested that AFB1 , HBV and HCV were as- sociated closely with human HCC , and these three factors might play a synergistic role in the oncogenesis of HCC.
出处
《中山医科大学学报》
CSCD
1996年第1期20-23,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
关键词
肝肿瘤
肝炎病毒
黄曲霉毒素
分析
liver neoplasms/genetics
aflatoxin/analysis
hepatitis B virus/genet- ics
hepatitis C virus/genetics
gene ,viral
