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反向遗传学技术及其应用 被引量:9

Reverse Genetics Technique and Its Application
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摘要 反向遗传学是直接从遗传物质入手来研究生命现象与规律的,其相应的操作技术已在生命科学研究的各个领域显示出了广泛的应用前景。文章从拯救病毒和创造新型病毒、负股RNA病毒的研究、动植物基因功能的研究以及利用RNA干扰使基因沉默等方面,对反向遗传学技术的应用进行了评述,并对其前景进行了阐述。 Reverse genetics investigates the phenomena and the law of life through direct manipulation of genetic material. Reverse genetics technique is showing extensive perspective in variety of fields of life science in which that has been used. The review summarized the application and perspective of reverse genetics technique in many aspects such as reviving virus, creating new virus, researching negative RNA virus and function of animals and plants gene, using RNA interference for gene silencing.
出处 《医学综述》 2006年第6期323-325,共3页 Medical Recapitulate
关键词 反向遗传学技术 拯救病毒 基因功能 RNA干扰 负股RNA病毒 Reverie genetics technique Revive virus Cene function RNA interference Negative Stranded RNA virus
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