摘要
目的采用PCR方法检测非霍奇金淋巴瘤的TCRβ基因重排,对贵州地区部分以往通过形态学和免疫学表现诊断的非霍奇金淋巴瘤重新在分子水平进行分析,探讨我省以往非霍奇金淋巴瘤诊断中存在的问题以及免疫受体蛋白基因重排在T-NHL和B-NHL的诊断和鉴别诊断中的价值。方法采用PCR方法,使用TCRβ引物,以 5例淋巴结反应性增生病例作为阴性对照,检测63例NHL患者TCRβ基因克隆性重排情况,其中T-NHL33例,B- NHL30例。结果 TCRβ基因重排在T-NHL中检测阳性26例,阳性率66．7％(22／33),在B-NHL中检测阳性7 例,阳性率23．33％(7／30)。经统计学处理TCRβ基因重排在T、B-NHL中的检出率有显著性差异(P<0．05)。结论 PCR方法检测NHL中TCRβ基因重排具有敏感、特异的优点,在T-NHL和B-NHL的的诊断和鉴别诊断中具有重要价值。可作为淋巴细胞来源的良、恶性病变的一种辅助诊断手段。
Objective To detect the application of TCR-β gene rearrangement in diagnosis of non-Hodgkin lymphoma by PCR technique, and to analyse some cases of non-Hedgkin lymphoma , which were diagnosed previously by morphological and immunophenotypic characteristic, at molecular level. To investigate previous problems in diagnosis of non-Hedgkin lymphoma in Guizhou province and significance of detecting gene rearrangement in diagnosis and differential diagnosis of T and B-NI-IL. Methods Take the PCR technique and TCR-β primers with 5 case lymphonode reactive hyperplasia as the negative contrast. Detect 63 eases NHL patients＇ TCR-β clonal gene rearrangement. Results TCR-β gene rearrangement was detected in 26 of 33 cases of T-NHL （78.78%）, and revealed in 7 of 30 cases of B-NHL（ 23.33% ）. Statistical analysis showed that there was significant difference（ P 〈 0.05） between the T and B-NHL. Conclusions PCR technique for TCR-β gene rearrangement is a sensitive , specific detection method , which is significant for diagnosis and differential diagnosis on T and B-NHL. The detecting of TCR-β gene rearrangement can be taken as a ancillary method to distinguish benign from malignant proliferation of lymphnode tissues.
出处
《合肥医学院学报》
2005年第6期521-523,共3页
Journal of Zunyi Medical University
基金
国家自然科学基金资助(NO:30160030)
