摘要
研究目的探讨反复呼吸道感染（ＲＲＴＩ）患儿血清微量元素含量，外周血Ｔ细胞亚群的变化及其意义。研究方法ＲＲＴＩ患儿９２例，男５８例，女３４例。４２名健康儿童为对照组。取清晨（７时～９时）空腹静脉血（肝素抗凝）（３～５）ｍｌ。血清微量元素谱测定采用Ｓｐｅｃｔｒａｓｐａｎ－Ｖ型三电极等离子原子发射直读光谱仪（ＤＣＰ－ＡＥＳ），同时测定１２种微量元素含量，如锌（７ｎ）、锰（Ｍｎ）、铝（Ａｌ）、镉（Ｃｄ）、铁（Ｆｅ）、铅（Ｐｂ）、铜（Ｃｕ）、镍（Ｎｉ）、钛（Ｔｉ）、钴（Ｃｏ）、锂（Ｌｉ）、铬（Ｃｒ）等。Ｔ细胞正群测定采用抗人单克隆抗体致敏红细胞花环法，Ｔ细胞增殖功能测定采用３Ｈ－ＴｄＲ掺入法。结果ＲＲＴＩ患儿血清微量元素Ｚｎ、Ｐｂ、Ｎｉ、Ｃｏ、Ｌｉ、Ｆｅ、Ｔｉ明显低于正常对照组。外周血Ｔ细胞亚群、细胞百分率、比值及Ｔ细胞增殖功能显著低于正常对照组，血清ＩｇＧ也低于对照组。
Objectives To search for the levels of the serum microelements, the measurement and significance of T cell subgroup of peripheral blood in child patients with recurrent respiratory tract infection (RRTI).Methods There were 92 children (58 males and 34 females)with RRTI in RRTI group,ahd 42 healthy children in control group. The 3 ￣ 5ml venous blood of them was drawn at 7 ￣ 9 0' clock in the morning (anticoagulated by heparin). The serum microelement spectrum was determined by spectraspan -V type of DCP-AES and 12 kinds of micro elements (Zn, Mn, Al, Cd, Fe, Pb, Cu, Ni, Ti, Co, Li and Cr) were determined at the same time. T cell subgroup and T cell proliferation function were determined respectively by erythrccyte-antibody rosette and 3H-TdR incorporation methods.Results The levels of serum microelements, Zn, Ph, Ni, Co, Li, Fe and Ti in RRTI group are lower than that in control group. The percentage of T-cell subgroup in peripheral blood, CD3+, CD4+, thevalue of CD4+/CD8+,T cell proliferation function and the levels of serum IgG in RRTI group are lower than that in control group.Conclusions There are extensive injuries of immunologic function in child patients with RRTI. Lacks of microelements should play an important role in occurrence and development of RRTI.
出处
《实用儿科临床杂志》
CAS
CSCD
1996年第1期6-9,共4页
Journal of Applied Clinical Pediatrics
关键词
呼吸道感染
微量元素
血清
T细胞亚群
儿童
recurrent respiratory tract infection
microelement
T cell subgroup
Child
