摘要
应用已克隆的鸡痘病毒（ＦＰＶ）２８２Ｅ＿４株基因组ＴＫ基因，在ＮｃｏⅠ部位引入痘苗病毒Ｐ７．５启动子和Ｐ１１启动子控制下的大肠杆菌ＬａｃＺ基因，经酶切鉴定，成功地构建了用于ＦＰＶ重组的载体质粒。与亲本毒株在鸡胚成纤维细胞内共转染，产生了表达ＬａｃＺ基因的重组鸡痘病毒。经传代筛选、纯化，重组病毒较稳定。实验结果表明，以ＴＫ基因构建的重组载体质粒可用于外源基因的重组表达研究。
Vaccinia virus P7.5 promoter and the E.coli LacZ gene driven by thevaccinia virus P11 promoter,were inserted into the Nco I site of TK gene fowlpoxvirus(FPV)282E_4 genome.The resulting plasmid,pFBLacZ,w identified by diges-tion with restriction endonucleases, and used to generate recombinant FPV by homolo-gous recombination in chicken embrvo fibroblast(CEF).The FPV recombinant can ex-pressed β-galactosidase from the E.coli LacZ gene,and could be easily plaque-purifiedand amplified.The result showed that the transfer vector plasmid constructed with FPVTK genes can be used to express foreign gene in FPV recombinant.
出处
《中国兽医学报》
CAS
CSCD
1996年第3期234-238,共5页
Chinese Journal of Veterinary Science
基金
国家自然科学基金
