摘要
目的研究靶蛋白P27和SKP2蛋白在肝细胞肝癌组织中的表达及与肝细胞肝癌各项临床病理指标的关系。方法用免疫组化二步法检测P27和SKP2蛋白在48例肝细胞肝癌、20例肝硬化及16例正常肝组织中的表达。结果48例肝癌组织中P27的阳性表达率(45.8%)显著低于肝硬化组织(80.0%)和正常肝组织(75.0%)。P27阳性表达与组织分化程度(P<0.01)、转移(P<0.05)、肿瘤大小(P<0.05)密切相关。48例肝癌组织中的SKP2的阳性表达率(33.3%)显著高于肝硬化组织(阴性表达)和正常肝组织(阴性表达),SKP2在肝细胞肝癌中的表达与组织分化程度(P<0.01)、转移(P<0.05)密切相关。肝细胞癌中SKP2的表达与P27表达呈负相关(r=-0.562,P<0.01)。结论肝细胞肝癌中SKP2的表达与P27的降解有关,P27的低表达可能与肝细胞肝癌的发生发展有关。SKP2的表达提示预后不良。
Objective To investigate the expression of targeted protein P27 and SKP2 protein in hepatocellular carcinoma and their relationship with clinicpathologic characters, and the correlation between expression of SKP2 and P27 in hepatocellular carcinoma. Methods The expression of P27 and SKP2 were evaluated in 48 human hepatocellular carcinoma tissues, 20 liver cirrhosis tissues as well as 16 normal liver tissues using immunohistochemistry （ two steps methods）. Results The expression rate of P27 in hepatocellular carcinoma （45. 8 %） was significantly lower than that in liver cirrhosis tissues （80.0 % ） and normal tissues （75.0%）. The expression of P27 was correlated with histological differentiation （P〈0. 01）, metastasis （P〈 0. 05）, tumour size （P〈0. 05）. The expression rate of SKP2（33. 3 % ） in hepatocellular carcinoma was significantly higher than that in liver cirrhosis tissues（negative expression） and normal liver tissues（negative expression）. The expression of SKP2 was closely correlated with differentiation （P〈0. 01 ）, metastasis （P 〈 0. 05）. The expression of SKP2 was negatively correlated with the expression of P27 （r = - 0. 562, P〈0.01 ）. Conclusion The degeneration of P27 may be correlated with the expression of SKP2,the lower expression of P27 may be correlated with the development of HCC, and the expression of SKP2 indicates the poor prognoss.
出处
《华中医学杂志》
CAS
2005年第5期390-391,共2页
Central China Medical Journal
