摘要
AIM： To investigate the roles of lymphocytes in the development of dextran sulfate sodium-induced colitis. METHODS： Using various doses of dextran sulfate sodium （DSS）, we induced colitis in wild-type B6 control and Rag-1 knockout （H-2b haplotype） mice, and evaluated the colitis in terms of symptomatic and histologic parameters, such as weight loss, survival, severity of diarrhea, shortage of colon length and histological changes. Symptomatic parameters were checked daily and histological changes were scored. RESULTS： Although development of colitis in Rag-1 knockout mice treated with high dose （5%） of DSS was comparable to that in B6 control mice, colitis progression was much more tolerable in Rag-1 knockout mice compared to than in B6 mice treated with low dose （1.5%） DSS. Symptomatic parameters as well as histopathologic changes were improved in Rag-1 knockout mice. CONCLUSION： These results indicate that the presence of lymphoo/tes contributes to colitis progression at low dose of DSS stimulation. Lymphoo/tes may play roles as an aggravating factor in DSS-induced colitis.
瞄准：在葡聚糖硫酸盐的发展调查淋巴细胞的角色导致钠的大肠炎。方法：用葡聚糖硫酸盐钠(决策支持系统)的各种各样的剂量，我们在野类型的 B6 控制和 Rag-1 大美人( H-2b haplotype )导致了大肠炎鼠标，并且评估了大肠炎以征兆并且 histologic 参数例如重量损失，幸存，腹泻的严厉，冒号长度和组织学的变化的缺乏。征兆的参数每天被检查，组织学的变化被获得。结果：尽管在 Rag-1 猛烈老鼠的大肠炎的开发与决策支持系统的高剂量(5%) 对待比得上在 B6 控制老鼠，大肠炎前进在 Rag-1 猛烈老鼠与相比是更可容忍的比在与低剂量(1.5%) 对待的 B6 老鼠决策支持系统。征兆的参数以及组织病理学说的变化在 Rag-1 猛烈鼠标被改进。结论：这些结果显示淋巴细胞的存在在决策支持系统刺激的低剂量贡献大肠炎前进。淋巴细胞可以在导致决策支持系统的大肠炎作为一个加重的因素起作用。
基金
Supported by Hallym University Research Fund, 2004, No.HRF-2004 -44
