摘要
Osmotic stress caused by dehydration or high-salt conditions poses a major environmental challenge for plant growth and development. Many studies have shown that protein kinases are important regulators during osmotic stress in plants. In the present study, we describe GmAAPK, a soybean （Glycine max （L.） Merr.） gene coding for a putative serine/threonine protein kinase （abscisic acid （ABA）-activated protein kinase （AAPK））. The cDNA for GmAAPKis 1 409 bp long and contains a single long open reading frame representing a complete coding region of 359 amino acids. GmAAPKwas mapped onto the D1a＋Q linkage group. Its transcripts are expressed in all tissues, but at high levels in the cotyledon. GmAAPKmRNA was induced by polyethylene glycol, ABA, Ca^2＋ and Na^＋, but not cold （4℃） treatments in soybean leaves. The results suggest that GmAAPK may participate in the regulatory process during osmotic stresses in soybean.
Osmotic stress caused by dehydration or high-salt conditions poses a major environmental challenge for plant growth and development. Many studies have shown that protein kinases are important regulators during osmotic stress in plants. In the present study, we describe GmAAPK, a soybean （Glycine max （L.） Merr.） gene coding for a putative serine/threonine protein kinase （abscisic acid （ABA）-activated protein kinase （AAPK））. The cDNA for GmAAPKis 1 409 bp long and contains a single long open reading frame representing a complete coding region of 359 amino acids. GmAAPKwas mapped onto the D1a＋Q linkage group. Its transcripts are expressed in all tissues, but at high levels in the cotyledon. GmAAPKmRNA was induced by polyethylene glycol, ABA, Ca^2＋ and Na^＋, but not cold （4℃） treatments in soybean leaves. The results suggest that GmAAPK may participate in the regulatory process during osmotic stresses in soybean.
基金
Supported by the National Natural Science Foundation of China （30392100 and 30370132） and the Hi-Tech Research and Development （863） Program of China （2002AA211051 ）.
