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超顺磁性氧化铁标记大鼠骨髓基质细胞经门静脉移植MRI活体示踪的研究 被引量：16

In vivo MR imaging of nanometer magnetically labeled bone marrow stromal cells transplanted via portal vein in rat liver
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摘要 目的观察1.5T场强MRI联合动物专用线圈是否可以活体示踪经门静脉移植的纳米级超顺磁性氧化铁颗粒标记的骨髓基质细胞(bonemarrowstromalcellsBMSCs),为介入性门静脉骨髓基质细胞移植治疗终末期肝脏疾病的研究提供进一步的依据。方法供体大鼠5只,梯度密度离心分离BMSCs,纳米级超顺磁性氧化铁颗粒和脂质体转染BMSCs,体外经普鲁士蓝染色和HE染色确定细胞标记率。受体大鼠15只,分为5组,分别为对照组和纳米级超顺磁性氧化铁颗粒标记的骨髓基质细胞经门静脉移植入正常大鼠肝脏后2h、3d、7d及2周组。1.5T场强MRI联合动物专用线圈行T1W、T2W和T2序列扫描,观察肝脏信号改变情况,与对照组比较,并且与组织切片对照。结果纳米级超顺磁性氧化铁颗粒和脂质体转染BMSCs,细胞标记率>95%。经门静脉移植入正常大鼠肝脏后,T2序列扫描显示经标记的BMSCs在肝内显示弥漫性的结节性低信号影,移植后2h到2周均可见到细胞在受体肝脏内存在,组织学切片显示信号缺失部位与铁颗粒标记细胞相一致。结论纳米级超顺磁性铁氧体颗粒标记的大鼠BMSCs经门静脉移植后可以通过1.5T场强行MRI活体示踪,为临床干细胞移植的应用提供可行的示踪方法。 Objective To evaluate in vivo magnetic resonance imaging with a conventional 1.5-T system for tracking of intra-portal vein transplantation nanometer magnetically labeled BMSCs in rat liver. Methods BMSCs were isolated from 5 SD rats bone marrow with the density gradient centrifugation method. Then BMSCs were labeled with nanometer superpara-magnetic iron oxide and transfection agent. Cell labeling efficiency was assessed with determination of the percentage of Perls Prussian blue stain. Then BMSCs transplanted into normal rats＇ livers via portal vein. The receipts were divided into 5 groups ,including sham control.2 h .3 d.7 d and 2 w after transplantation. Follow-up serial T1WI、T2WI and T2 ＊ -weighted gradientecho MR imaging were performed at 1.5 T MRI system. MR imaging findings were compared with histology. Results Cell labeling efficiency was more than 95% by Perls Prussian blue stain. After transplantation of labeled BMSCs via portal vein, livers had diffuse granular signal intensity appearance in T2 ＊ WI MRI. Cells were detected for up to 2 w in receipts＇ livers. At histologic analysis, signal intensity loss correlated with iron-loaded cells. Conclusion MR imaging could aid in monitoring, of magnetically labeled BMSCs administered via portal vein in vivo.
作者 王平 王建华 颜志平 胡美玉 徐鹏举 周梅玲 严福华 FAN Sheung-tat LUK John-m
机构地区 复旦大学附属中山医院放射科 复旦大学附属中山医院中心实验室 香港大学肝脏疾病研究中心、香港大学医学院外科学系
出处 《中华放射学杂志》 CAS CSCD 北大核心 2006年第2期133-138,共6页 Chinese Journal of Radiology
基金 香港大学医学院营养与肝肿瘤相关的领先研究基金资助项目(HKU7320/02M) 香港研究基金会基金~~
关键词 干细胞 磁共振成像 细胞移植 门静脉 动物 实验 Stem cells Magnetic resonance imaging Cell transplantation Portal vein Animals, laboratory
分类号 R445.2 [医药卫生—影像医学与核医学]
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参考文献14

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二级参考文献23

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共引文献70

同被引文献222

引证文献16

二级引证文献55

中华放射学杂志
2006年 第2期

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