摘要
目的研究原发性胆囊癌组织中VEGF(vascular endothelial growth factor,血管内皮生长因子)、COX-2的表达与血管形成的关系及预后意义。方法应用免疫组织化学SP法对64例胆囊癌组织COX-2、VEGF表达和MVC进行检测。结果 COX-2和VEGF阳性表达率分别为72％和 55％。64例胆囊癌MVC平均为(57±14)个／HP。MVC与胆囊癌分化程度、Nevin分期和淋巴结转移密切相关(t=2．948,t=5．102,t=7．329,P<0．05)。VEGF在中低分化型和有淋巴结转移胆囊癌中的表达显著高于高分化型和淋巴结未转移者(x2=5．752,x2=10．093,P<0．05)。COX-2在Nevin分期S4-S5和有淋巴结转移的胆囊癌中的表达显著高于分期为S1-S3和淋巴结未转移者(x2=6．886, x2=12．882,P<0．05)。COX-2和VEGF表达与MVC值之间具有显著相关性(r=0．268,x2=4．608, P<0．05,t=5．424,P<0．05)。COX-2、VEGF的表达与患者的预后有关(x2=8．276,x2=6．656, P<0．05),结论 COX-2、VEGF和MVC是反映胆囊癌生物学行为的重要参数。COX-2高表达与胆囊癌的血管生成密切相关,其可能机制之一是促进VEGF的表达。
Objective To investigate the relationships between the expression of cyclooxygenase-2 （COX-2） , vascular endothelial growth factor （VEGF） and the degree of vascularization, clinicopathological feature, survival time of the patients with gallbladder carcinoma. Methods Routine paraffin-embeded sections of gallbladder carcinoma tissues in 64 cases were evaluated for COX-2, VEGF expression and MV by the streptavidin-peroxidase complex immunohistochemical method. Results COX-2, VEGF immunoreactivity were observed in 72% , and 55% cases, respectively. The average MVC was （57 ± 14 ）/ HP. The status of MVC was closely correlated with Nevin staging, tumor differentiation and lymph node metastasis （P 〈 0.05 ）. Increased VEGF expression was significantly correlated with tumor differentiation （ poor, moderate 〉 well differentiated, P 〈 0. 05 ）. Clinical stages had no relation with expression of VEGF （P 〉 0. 05 ）. There was a positive correlation between COX-2 expression and clinical stages. The positive rate of COX-2 was higher in cases of Nevin stages S4-S5 （82%） with lymph node metastasis than in those of Nevin stages S1 -S3 （ 50% ） and without metastasis （ P 〈 0. 05 ）. The expression of COX-2 did not vary with differentiation（ P 〉 0.05 ）. The expression of VEGF and COX-2 was significantly correlated with that of MVC （t=5.424,P 〈0.05; x^2 -4.608,P 〈0.05）. Patients with COX-2, VEGF positive tumors had a significantly shorter survival time than those with COX-2, VEGF negative tumors （ x^2 = 8. 276, x^2 = 6. 656, P 〈 0.05）. Conclusions Augmented tumor neovascularization induced by VEGF may be one of the effects of COX-2 responsible for poor prognosis in human gallbladder carcinoma.
出处
《中华普通外科杂志》
CSCD
北大核心
2006年第2期138-141,共4页
Chinese Journal of General Surgery
关键词
胆囊肿瘤
新生血管化
病理性
内皮生长因子
Gallbladder neoplasms
Neovascularization, pathologic
Endothelial growth factors
