摘要
目的探讨中国人对氧磷酶基因-162A/G-、909C/G遗传多态性与冠心病的关系。方法应用聚合酶链反应对冠心病患者和正常对照者对氧磷酶1基因酶切位点的限制性片段长度多态性进行分析。结果对氧磷酶1-162A/G多态性位点在正常对照组中AA、AG、GG基因型频率及A、G等位基因频率分别为0.047、0.203、0.750和0.148、0.852,冠心病组与对照组基因型分布总体构成比差异无显著性(P>0.05),冠心病组与对照组等位基因频率分布差异也无显著性(P>0.05)。对氧磷酶1-909C/G多态性位点在正常对照组中CC、CG、GG基因型频率及C、G等位基因频率分别为0.258、0.523、0.219和0.520、0.480,冠心病组与对照组基因型频率和等位基因频率差异未见显著性(P>0.05)。冠心病不随两个多态性位点危险基因型个数的增加而发病率呈上升趋势(P>0.05)。结论未发现对氧磷酶1-162A/G和对氧磷酶1-909C/G基因多态性与中国人群冠心病相关联,对氧磷酶1-162A/G和对氧磷酶1-909C/G基因多态性对冠心病的影响无协同性。
Aim To study the association between the polymorphism in the promoter region in human paraoxonase （PON1） gene and coronary heart disease（CHD）in Chinese population. Methods The genotype and allele frequeney of paraoxonasel gene polymorphism were assayed by polymerase chain reaction （PCR）-restrietion fragment length polymorphism （RFLP）. Results The frequencies of PONl-162A/G genotype and allele in control were 0.047, 0.203, 0.750 and 0.148, 0.852, respectively. There was no significant difference in genotype and allel frequency between control and CHD group. The frequencies of PON1-909C/G genotype and aUele in control were 0.258, 0.523, 0.219 and 0.520, 0.480, respectively. There wes no significant difference in genotype and allel frequency between control and CHD group. Increasing number of hazardous genotype of PON1 was not associated with CHD. Conclusions These results suggested that PON1-162MG,PON1-909C/G gene polymorphism were not associated with CHD in Chinese population. There was no cooperative effect among these hazardous genotype with CHD.
出处
《中国动脉硬化杂志》
CAS
CSCD
2005年第5期619-622,共4页
Chinese Journal of Arteriosclerosis
关键词
内科学
对氧磷酶
启动子
动脉粥样硬化
冠心病
基因型
等位基因频率
Paraoxonase
Atherosclerosis
Coronary Heart Disease
Polymorphism
Hazardous Genotype
Cooperative Effect
