摘要
目的研究儿童终丝中神经干细胞的分布,为脊髓栓系综合征(TetheredCordSyndrome,TCS)的治疗提供新的策略。方法取TCS患者内终丝近远端40例,用免疫组织化学的方法进行染色,观察巢蛋白(Nestin)表达。结果终丝的近远端均有Nestin阳性细胞,近端22例阳性表达占15例,阳性率为68.1%,远端18例阳性表达有10例,阳性率为55.6%;χ2检验显示:终丝近远端阳性表达无差异。结论TCS患者终丝中存在Nestin阳性细胞,这为从TCS患者终丝中诱导、分化神经干细胞,对TCS患者进行神经干细胞治疗提供了理论基础,为TCS和其它中枢神经系统疾病患者进行神经干细胞治疗提供新的材料来源。
Objective To study the distribution of neural stem cells in the ilium terminale of children in order to provide a new measures against Tethered Cord Syndrome. Methods forty selected specimens of the distal and proximal intradural ilium terminale from the people suffered from the tethered cord syndrome were used for the observation on the expression of nestin by the means of immunohistochemistry. Results Nestin positive cells were found in the distal and proximal ilium terminale, fifteen out of twenty-two specimens of the proximal intradural ilium terminale showed positive expression of nestin,the positive rates of nestin were 68.1%,ten out of eighteen specimens of the distal ilium terminale showed positive expression of nestin,the positive rates were 55.6%.Chi-square test indicates that there was no significant difference of nestin expression between distal and proximal intradural ilium terminale. Conclusions There are＇ nestin positive cells in the ilium terminale of tethered cord syndrome, which will provide a theory base about the neural stem ceils applies to therapy the tethered cord syndrome as well as inducement and differentiation of neural stem ceils derived from the ilium terminale of patient.h is also to study the distribution of neural stem ceils in the ilium terminale of children in order to provide a new measure against tethered cord syndrome or other diseases of central nerve svstem.
出处
《临床小儿外科杂志》
CAS
2005年第6期425-429,共5页
Journal of Clinical Pediatric Surgery
关键词
干细胞
神经系统
Stem cells
Nervous System
