摘要
目的:优选一种适用献血员梅毒检测的试验方法。方法:采用梅毒特异性抗体的双抗原夹心法(ELISA)对献血员进行抗体检测,并与RPR法和TRUST法检测结果进行比较,3种方法检测结果有反应性的标本,再用TPHA法进行确证。结果:ELISA法有反应性率1.69%(184/10884),RPR法有反应性率1.23%(134/10884),TRUST法有反应性率1.30%(141/10884);ELISA法与TPHA法符合率97.8%(180/184),RPR法与TPHA法符合率71.2%(131/184),TRUST法与TPHA符合率75.5%(139/184)。进行统计学处理,ELISA法与RPR法、TRUST法具有非常显著性差异。ELISA法与TPHA法无显著性差异。结论:ELISA法优于TRUST法和RPR法具有较高的灵敏度和特异性,适合献血员的梅毒项目的优选试验方法,有利于控制梅毒的输血传播途径。
Objective：To select a suitable method for the detection of anti-TP antibody in blood donors. Methods：Anti-TP antibody was detected by ELISA of double sandwich, and compare with the results of RPR and TRUST. Then TPHA method was employed to confirm the reactive samples. Results：The reactive rate of ELISA was 1.69%（184/10 884）,and RPR was 1.23%（134/10 884）, while TRUST was 1.30%（141/10 884）;Thc coincidental rate between ELISA and TPHA was 97.8%（180/184）,between RPR and TPHA was 71.2%（131/ 184）,while that between TRUST and TPHA was 75.5%（139/184）. After statistical analysis,the difference between ELISA and RPR was significant,and so was that between ELISA and TRUST,but there was no obvious difference between ELISA and TPHA. Conclusion： ELISA is better than TRUST and RPR,and suitable for the detection of anti-TP antibody in blood donors. Because of its high sensibility and specificity,it is helpful to control the route of syphihtic transmission in blood transfusion.
出处
《现代医药卫生》
2006年第6期803-804,共2页
Journal of Modern Medicine & Health
