摘要
目的探讨云南地区白细胞介素1(IL-1)基因型分布特点及其与幽门螺杆菌(HP)感染、定植密度间的关系。方法对符合研究标准的患者,进行HP感染及HP定植密度的评定;用PCR-RFLP检测外周血的IL-1B-511、IL-1B+3954、IL-1RN的基因型。结果IL-1B-511基因型中,C/C24例(17.3%)、C/T75例(54.0%)、T/T40例(28.8%);IL-1B+3954基因型中,C/C132例(95.0%)、C/T7例(5.0%)、无T/T;IL-1RN位点L/L与1/2基因型各占111例(79.9%)、27例(19.4%)、2/2基因型1例。IL-1B-511含T基因型是DU患者HP感染的易感因素,OR为6.1。在≥60岁亚组中,IL-1B-511含T基因型是HP定植密度程度的危险因素,OR为8.0。结论东西方非胃肿瘤患者人群IL-1基因型分布存在差异,为研究东西方人群HP感染相关临床结局类型差异的原因提供线索。IL-1B-511含T基因型与DU患者HP感染率高及与易形成重度HP感染相关。
Objective To study the distrbuition characteristics of interleukin-1 genotype and the relation between interleukin-1 genotype and the infection and density of helicobacter pylori in Yunnan region. Methods The infection and density of helicobacter pylori were investigated from the gastric mucous membrane samples ; The genotypes of interleukin-1 beta-511 （ IL-1B^- 511 ）, interleukin-1 beta ＋ 3954 （ IL- 1 B^＋3954） , interleukin-1 receptor antibody （IL-1RN） from peripheral blood were determined by the polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. Results Among interleukin-1 beta-511 （IL-1B^-511 ） genotypes, There were C/C genotype 24 cases （ 17.3% ） ,C/T 75 cases （54.0%） ,T/T 40 cases （28.8%） ; among interleukin-1 beta ＋ 3954（ IL-1B^＋3954 ） genotypes, There were C/T genotype 7 cases （ 5. 0% ）, C/C genotype 132 cases （95.0%）, no T/T ; Interleukin-1 receptor antibody （IL-1RN） genotypes, There was 1/4 ,2/2 genotype each 1 case,1/1 110 cases （79.2%） ,1/2 27 cases （ 19.4% ）. IL-1 B^-511 ＊/T genotype is predisposing factor for helicobacter pylori infection, OR value is 6.1. In patients with age above 60 years old, IL-1 B^-511 ＊/T genotype is risk factor for helicobacter pylori density,OR value is 8.0. Conclusion There was difference in the clinical results related to helicobacter pylori-infection between people in east and in west. IL-1B^-511 ＊/T genotype was related to the extra high H. pylori detection rate in patients with DU, this genotype could easily result in severe infection of helicobacter pylori.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2006年第2期111-113,共3页
Chinese Journal of Laboratory Medicine
基金
云南教委基金资助项目(OZQY131)
