摘要
本文采用单柱离子色谱法测定ＰＯ_４^（３－）ｋ别以对羟基苯甲酸、邻羟基苯甲酸和邻苯二甲酸为淋洗液，考查了淋洗液酸度变化对ＰＯ_￥^（３－）、Ｃｌ－、ＮＯ_３~－、ＳＯ_４^（２－）保留时间的影响，并根据上述四种离子和淋洗剂表观电荷随ｐＨ值的变化关系解释了ＰＯ_４^（３－）与Ｃｌ、ＮＯ３、ＳＯ_４^（２－）保留时间随淋洗液ｐＨ值变化曲线的差异．三类淋洗液中以对羟基苯甲酸为淋洗液测定ＰＯ_４（３－）最好，且淋洗液的最佳ｐＨ值为７．０、浓度为１．０ｍｍｏｌ／Ｌ。ＰＯ_４^（３－）的检出限为０．７４μｇ／ｍＬ．相对标准偏差为２．０５％．线性范围为０．７４—１００μｇ／ｍＬ．应用于饮料中ＰＯ_４（３－）的测定，结果良好。
Determination of orthophosphate by single-column ion chromatography was described.p-hydroxybcnzoic acid,salicylic acid and phthalic acid tvers used as eluent, respectively.The influence of eluent PH on retention time of PO_4￣(3-), Cl-, NO_3￣-,and SO_4￣(2-) was investigated.Variational curve of retention time for PO_4￣(3-) versus eluent PH was different from Cl-, NO_3￣- and SO_4(2-). This was explained by variation of apparent charge for PO_4￣(3-), Cl-, NO_3￣-, SO_4￣(2-) andcluent versus PH. The potimum PH and concentration of p-hydroxybenzoic acid eluent were 7.0and 1.0mmol/L, respectively. The detection limit for PO_4￣(3-)was 0.74μg/mL.The relative standard deviation was 2.85%. The calibration curve was liner in the range of 0.74- 100μg/ml. The method has ho uest in analysis of PO_4￣(3-) in beverages with satisfactory results.
出处
《哈尔滨师范大学自然科学学报》
CAS
1996年第1期58-61,共4页
Natural Science Journal of Harbin Normal University
关键词
离子色谱法
磷酸根
测定
Ion chromatography,Orthophosphate
