摘要
用组织化学、亲和组织化学方法研究了黄胫小车蝗OedaleusinfernalisSaussure交配前后受精囊内含物的化学组成。结果表明,黄胫小车蝗受精囊内含物有蛋白质、脂类和碳水化合物。交配前后黄胫小车蝗受精囊腔及腺细胞中蛋白质、碳水化合物的含量有较大差异,交配后的含量明显高于交配前的,说明交配活动启动了受精囊腺细胞的分泌,使受精囊腔及受精囊管中积聚大量的碳水化合物及蛋白质。交配前后受精囊脂类含量没有明显变化。用亲和组织化学方法对交配后受精囊进行染色分析,表明受精囊腔内含物的碳水化合物、蛋白质主要以糖蛋白形式存在,糖残基主要有半乳糖、甘露糖及α_葡萄糖。
The contents of the spermatheca of the grasshopper Oedaleus infernalis Saussure were assayed with histochemistry and affinity-histochemistry methods. Spermathecae before and after mating were stained with PAS, bromophenol blue and Sudan black B. The results showed that the contents of spermatheca included carbohydrates, proteins and lipids, and there were clear differences in these contents in gland cells and spermathecal lumen between spermathecae before and after mating. These contents were in higher content in spermathecae after mating than in those before mating. This suggested that mating stimulated secretion by gland cells and resulted in accumulation of carbohydrates and proteins in the spermathecal lumen and duct. However, the content of lipids shown with Sudan black B staining showed no distinct differences in spermathecae before and after mating. Affinity-histochemistry results indicated that carbohydrates and proteins in spermathecae were present most in the form of glycoproteins, and oligosaccharides of these glycoproteins consisted mainly of mannose, galactose and glucose.
出处
《昆虫学报》
CAS
CSCD
北大核心
2006年第1期38-44,共7页
Acta Entomologica Sinica
基金
陕西省自然科学基金项目(2000SM22)
陕西师范大学重点项目
关键词
黄胫小车蝗
受精囊
内含物
组织化学
亲和组织化学
Oedaleus infernalis
spermatheca
content
histochemistry
affinity-histochemistry
