摘要
目的探讨乙酰肝素酶(Heparanase Hpa)在肝癌中的表达及其与肝癌临床病理特征的关系。方法分别采用RT-PCR和免疫组织化学法检测Hpa mRNA及Hpa蛋白在肝细胞肝癌、癌旁肝组织及肝癌细胞株中的表达。结果肝癌Hpa mRNA的表达显著高于癌旁及正常肝组织(P<0.05),肝癌组织中Hpa mRNA的表达与肿瘤包膜受侵、门脉受侵、肝内转移、TNM分期、复发有关(P<0.05)。肝癌Hpa蛋白的表达与包膜和门脉是否受侵、Edmondson分级、有无肝内转移、TNM分期及复发有关(P<0.05)。结论Hpa mRNA及蛋白在肝癌中高表达,并与肝癌的侵袭转移、复发、预后有关。
Objective The main aim of this investigation were to explore the expression of heparanase mRNA and protein in hepatocellular carcinoma （HCC） and the relationship between the expression of Hpa and clinical pathological features of HCC. Methods Reverse transcription polymerase chain reaction （RT-PCR） and immunohistochemical staining were used to detect expression of the heparanase mRNA and protein in normal hepatic tissues, liver cancer cell line, tumor tissues and adjacem tissues of hepatocellular carcinoma. Results The expression of Hpa mRNA and protein in HCC tissues was significantly higher than that in adjacent tissue and normal hepatic tissues（P〈0. 05）. It was correlated with capsular invasion, portal vein invasion, intrahepatic metastasis, TNM classification and recurrence of HCC（P〈0. 05）. Hpa protein expression was related with Edmondson-Steiner＇s classification（P〈0. 05）. Conclusion Overexpression of the heparanase mRNA and protein related to the invasion, metastasis, recurrence and prognosis of hepatocellular carcinoma.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2006年第2期80-82,136,共4页
Cancer Research on Prevention and Treatment
基金
河北省卫生厅科研基金资助项目(04029)
关键词
肝细胞肝癌
乙酰肝素酶
侵袭
转移
复发
Hepatocellular carcinoma
Heparanase
Invasion
Metastasis
Recurrence
