摘要
目的：探讨RapIDYeast Plus（RYP）系统在鉴定临床常见酵母中的作用。方法：将所试菌株在沙氏培养基上传代2次，然后30℃孵育24-48h后，用RYP系统进行试验。结果：150株临床分离株中有139株被正确鉴定到种的水平；有8株得到了具有2种或2种以上可能性的鉴定结果，而在使用了附加试验后都得出了正确的鉴定结果；有3株鉴定错误。RYP系统在不用附加试验的情况下鉴定的准确率达到92％，采用附加试验后鉴定的准确率达到98％。结论：RYP系统适用于临床微生物实验室的常规鉴定，在不需要特殊仪器的条件下，就能准确地鉴定临床上40多种酵母菌和酵母样菌。
Objective ：To study the role of RaplDYeast Plus （RYP） system in identifying common clinical yeasts. Methods.. The target strains were cultured and passaged twice on Sabouraud dextrose agar, and were fed to RYP system after 24-48 h incubation at 30℃. Results.. One hundred and thirty-nine of the 150 target strains Were identified to the level of species correctly, and 8 undetermined strains were confirmed by additional tests. It was found that 3 strains had been incorrectly identified by RYP system. The accuracy of RYP system was calculated as 92 % without additional tests and 98 % with additional tests. Conclusion.. RYP system is suitable for routine tests in clinical microbiological laboratory; it can accurately identify more than 40 kinds of yeasts and yeast-like bacteria in clinical practice.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2006年第2期215-216,共2页
Academic Journal of Second Military Medical University
