摘要
目的：探讨自制上海多器官保存液（SMO液）对肾皮质线粒体低温缺氧损伤的保护作用。方法：以HC-A液和UW液作对照，在犬肾低温保存各时间点，取皮质标本；通过电镜观察保存期间线粒体形态改变；差速离心法分离皮质线粒体，Clark氧电极法测定其活性，描记四态呼吸曲线，计算Ⅲ态、Ⅳ态耗氧速率、线粒体呼吸控制率（RCR）及磷氧比（P／O）。结果：低温保存期间，HC-A组肾皮质线粒体水肿、空泡样变明显重于SMO组和UW组，而后两者改变相似；随着低温保存时间延长，实验组和对照组肾皮质线粒体Ⅲ态呼吸耗氧速率、RCR和P／0均明显降低；保存1～3dHC-A组Ⅲ态呼吸耗氧速率显著低于SMO组（P〈0．05）；保存各时间点SMO组线粒体RCR和P／O显著高于HC-A组（P〈0．05），与UW组无明显差异。结论：SMO液对肾皮质线粒体低温缺氧损伤的保护作用比HC-A液强，与UW液相似。
Objective：To study the protective effect of self-designed Shanghai multi-organ preservation solution（SMO） on mitochondria function in canine kidney cortex during cold storage. Methods： Canine kidneys were perfused and stored with cold （0-4℃） SMO, HC-A and UW solution （the later 2 as control） separately. Ultrastructure alteration of mitochondria was observed by electron microscopy. Mitochondria were isolated from cortex homogenization by differential centrifugation and their respiratory function was measured polarographically with Clark oxygen electrode. The four-state curve of mitochondria respiration was drawn and the state Ⅲ and Ⅳ oxygen consumption rate, the respiration control proportion（RCR） and phosphatasel/oxygen ratio（P/O） were calculated. Results： We found that the mitochondria swelling and membrane disruption in HC-A group were more severe than those in SMO and UW group. The rate of oxygen consumption in state Ⅲ , RCR and P/O were obviously decreased in all groups with the prolongation of cold storage time. The rate of oxygen consumption in state Ⅲ in HC-A group was lower compared to that in SMO group during 1-3 d cold storage（P〈0.05）. The rate of oxygen consumption in state Ⅳ was slightly increased during the cold storage, but there was no statistical difference between HC-A and SMO group. RCR and P/O in SMO group were statistically higher than those in HC-A group （P〈0.05） and were similar to those of UW group. Conclusion： SMO solution has similar protective effect on cortex mitochondria function impairment induced by hypothermia and ischemia during cold storage as UW solution,and the effect is more potent than that of HC-A solution.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2006年第2期196-199,共4页
Academic Journal of Second Military Medical University
基金
上海市科委重点项目(024919006)~~
关键词
器官保存液
低温保存
缺氧
肾皮质
线粒体
保护作用
organ preservation solutions
cryopreservation
anoxia
kidney cortex
mitochondria
