摘要
目的：研究糖皮质激素受体β（GRβ）的稳定过度表达对激素调节人骨肉瘤细胞增殖分化作用的影响，探讨糖皮质激素受体口的生物学意义。方法：将GRβ在人骨肉瘤细胞中稳定过度表达，用不同浓度（10^-10～10^-6mol／L）的地塞米松处理稳定过度表达GRβ的细胞，用活细胞计数法和MTT法检测细胞的增殖，用标准酚比色法检测碱性磷酸酶（AKP）的活性。结杲与结论：在稳定过度表达GRβ的人骨肉瘤细胞中，糖皮质激素抑制细胞增殖和诱导细胞分化的作用均减弱；GRβ可能是GRα的内源性抑制因子。
Objective：To study the effect of glucocorticoid receptor beta overexpression on the regulatory effect of glucocorticoid on proliferation and differentiation of human osteosarcoma cell and to investigate the biological significance of glucocorticold receptor beta. Methods： Glucocorticoid receptor beta was stably transfected into human osteosarcoma cells and the stable transfectants were treated with different concentrations of dexamethasone （10^-10～10^-6mol/L）. Then cell proliferation was de- termined by MTT method and viable cell counting; alkaline phosphatase was detected by standard hydroxybenzene colorimetry. Results and Conclusion： In stable transfectants overexpressing glucocorticoid receptor beta, glucocorticoid-induced cell proliferation repression and cell differentiation are inhibited,indicating that glucocorticoid receptor beta may be an endogenous inhibitor of glucocorticoid receptor alpha.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2006年第2期186-188,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金(39870304)~~
关键词
受体
糖皮质激素
骨肉瘤
细胞增殖
细胞分化
receptors,glucocorticoid
osteosarcoma
proliferation
differentiation
