摘要
目的：研究葡萄糖、甘露糖、半乳糖对新生隐球菌荚膜相关基因CAP10启动活性的影响。方法：用含CAP10编码区上游5’侧翼序列（-951～0bp）和报告基因的重组体转化酵母，分别用不同浓度（10、20、40、60、80mg／ml）的葡萄糖、甘露糖、半乳糖对其进行干预，用ELISA方法检测CAT表达值，比较各组间CAT表达活性的不同。结果：不同浓度的葡萄糖和甘露糖对CAT的表达强弱无明显的影响；而不同浓度的半乳糖对CAT的表达强弱有明显的影响，而且这种正向诱导作用在实验所用的剂量范围内具有剂量依赖关系。结论：葡萄糖和甘露糖对CAP10启动活性无明显影响；半乳糖对CAP10的启动活性有明显的诱导作用，推测CAP10基因可能与新生隐球菌中半乳糖的代谢有关。
Objective：To study the effects of glucose, mannose and galactose on CAP10 promoter activity of Cryptococcus neoforrnans capsule-associated gene. Methods： Yeast cells were transfected with plasmid containing a 951 bp length of 5＇ upstream flanking sequence of CAP10 coding region and a reporter gene, chloramphenicol acetyl transferase （CAT） ; the transfectants were treated with different concentrations（ 10,20,40,60,80 mg/ml） of glucose, mannose and galactose. CAT activity was assessed by ELISA method and CAT activities of different groups were compared. Results： Different concentrations of glucose and mannose had no obvious influence on CAT activity; different concentrations of galactose had obvious influence on CAT activity and the influence was positively dependent with its dose within the experimental concentration range. Conclusion： Glucose and mannose have no obvious effect on the activity of CAP10 promoter; galactose has obvious inductive effect on activity of CAP10 promoter, suggesting that CAP10 gene might be related with galactose metabolism.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2006年第2期132-135,共4页
Academic Journal of Second Military Medical University
基金
国家自然科学基金(30270080)~~
