摘要
为了解婴儿先天性巨结肠（ＨＤ）与巨细胞病毒（ＣＭＶ）感染的关系，对１９例２～１８个月的ＨＤ患儿（经病理证实）的血清、尿和组织块用病毒分离和ＤＮＡ探针杂交方法进行ＣＭＶ检测。结果：１９例ＨＤ患儿分离ＣＭＶ的１９份尿标本中２份污染，在１７份尿标本中７份阳性，阳性率为４１．２％；正常儿尿标本阳性率为１４．４％（Ｐ＜０．０５）。１９例ＨＤ血清中，ＣＭＶＩｇＭ抗体２例阳性，阳性率为１０．５％；正常儿阳性率为８．０％（Ｐ＞０．０５）。１９例ＨＤ痉挛段组织块中无一例ＣＭＶ阳性，但６例ＨＤ痉挛段组织块ＣＭＶ－ＤＮＡ探针杂交全部阳性，其中２例强阳性，阳性率为１００％。说明婴儿ＨＤ与ＣＭＶ感染关系密切，ＣＭＶ感染可能是包括ＨＤ在内的一些先天性畸形的重要致畸因素。
To understand the relation between congenital megacolon and cytomegalovirus(CMV)infection in infants,nineteen infants with megacolon(confirmed by pathological examination)were studied.Their ages ranged from 2 to 18 months. CMV was detected in the blood,urine and pathological specimen by virus isolation and DNA needle hybridism. The results were as follows:two urine samples were contaminated, seven were postitive( 4 1.2%)V.S.14.4%positive in normal(P<0.05). In the 2 of 19 serum samples in HD children, CMV IgM antibodies were positive(10.5%),V.S. 8.0%in normal serum samples(P> 0.05%).In 19 spastic segment tissue masses of HD,none was positive in CMV detection, 6 were positive in CMV-DNA needle hybridism,2 strongly positive(100%).These indicate that HD is closely related to CMV infection. CMV infection may be an important teratological factor of some congenital malformations including HD.
出处
《中华小儿外科杂志》
CSCD
1996年第1期3-5,共3页
Chinese Journal of Pediatric Surgery
关键词
婴儿
先天性
巨结肠
巨细胞病毒
Hirschsprung's disease
Cytomegaloviruses
Cytopathogenic effect,viral
