摘要
目的观察MPTP(1-甲基-4-苯基-1,2,3,6-四氢吡啶)对C57BL/6J小鼠纹状体DA(多巴胺)、5-HT(5-羟色胺)等神经递质的释放节律性的影响。方法取120只SPF级C57BL/6 J小鼠,随机分为2组:MPTP组连续5 d腹腔注射MPTP-HCl 36mg/(kg.d),对照组注射生理盐水300μL。5 d后24 h内分6个时间点:ZT0、ZT4、ZT8、ZT12、ZT16、ZT20,取纹状体,用HPLC-ECD法检测各时间点2组纹状体中DA、5-HT的水平。结果MPTP组纹状体DA水平明显降低(P<0.05);对照组DA高峰出现在ZT0(即8:30 am),低谷在ZT16(即1:30 am),MPTP组DA的高峰和低谷出现的时相未发生改变。2组5-HT均无明显节律性释放。结论小鼠纹状体的DA水平呈节律性变化,5-HT无节律性变化,MPTP作用后对DA的节律性变化无影响。表明此模型纹状体DA的减少与DA释放节律性之间不存在必然联系。
Objective To investigate the effect of MPTP on the circadian oscillation of striatal dopamine （DA） release in C57BL/ 6J mice. Methods 120 C57BL/6J male mice were divided into 2 groups： MPTP group was administrated with 36 mg/（kg·d） MPTP- HCl for 5 consecutive days. Control group was treated with saline. After last injection, mice were sacrificed at six different time points during the 24 h period. The DA and serotonin （5 HT） levels in the striatum were deten＇nined by HPLC with electrochemical detection. Results DA level decreased significantly in MPTP group （P 〈 0.05 ）. The cbanges of DA levels in striatum of control mice during the 24 h period revealed a significant lower point at ZT16 （1：30 am） and a peak at ZT0 （8：30 am）. MPTP group showed the same circadian oscillation. There was no circadian oscillation of 5-HT levels in both groups. Conclusion MPTP did not affect the circadian oscillation of DA release, indicating the lack of interaction between DA decrcase and circadian oscillation of DA release.
出处
《首都医科大学学报》
CAS
2006年第1期66-69,共4页
Journal of Capital Medical University
基金
国家自然科学基金(30400148)
国家科技攻关计划子课题(200BA711A07-04)
北京市自然科学基金重点项目(7031002)
北京市科技新星项目(H020821400190)资助项目
