摘要
目的探讨α2b-IFN对PBMC内CD25的诱导及对HCV-RNA、抗-HCV转阴效果。方法用BSA法检测慢性丙肝患者治疗前后CD25表达水平。以α2b-IFN（3MU／d）治疗3个月为一疗程，共2个疗程，于治疗前后分别检测患者PBMC内HCV-RNA和血清HCV-RNA、抗-HCV。结果慢性丙肝患者α2b-IFN治疗后静息期、诱导期CD25表达水平分别为（3．44±0．77）％、（33．62±3．95）％，PBMC、血清内HCV-RNA和抗-HCV转阴率分别为42．31％（11／26）、57．69％（15／26）和65．38％（17／26），与对照相比差异有显著性（P〈0．01～P〈0．05）。结论α2b—IFN可诱导CD25表达，对PBMC内HCV-RNA具有肯定的治疗作用，其疗效优于常规治疗。
Objective To study the level of CD25 on surface of peripheral blood mononuelear cells （PBMC） and the effect of α2b-IFN on HCV-RNA in PBMC and anti-HCV in serum. Methods Before and after treament, the level of CD25 of patients with chronic hepatitis C was detected by biotin-streptavidin （BSA） and the eases were treated with α2b- IFN （3 MU/d）. The total course of the treatment of α2b-IFN was six months and per course of treatment was three months. Results Before and after treatment of α2b-IFN, the levels of CD25 in silence and inducement were （ 3.44 ± 0.77% ） and （33.62 ± 3.95 ）%. The negative rates of HCV-RNA in the PBMC and HCV-RNA, anti-HCV in serum were 42.31% （11/26） ,57.69% （15/26） ,65.38 % （17/26） , respectively. There was higher significantly difference between in treatment of α2b-IFN and controls （P 〈 0.01 - P 〈 0.05 ）. Conclusion CD25 could be induced by α2b-IFN during the treatment and better curative effect of α2b-IFN had been taken on the treatment of HCV-RNA in PBMC than that of the routine drugs.
出处
《中国微生态学杂志》
CAS
CSCD
2006年第1期55-56,60,共3页
Chinese Journal of Microecology
基金
安徽省教育厅自然科学基金重点项目(编号:2003kj027zd
