摘要
制备抗HIV-1gp41合成多肽gp41-5的单克隆抗体(mAb),为筛选抗HIV-1多肽及分析gp41的抗原表位提供有用工具。常规动物免疫、细胞融合、克隆化制备抗gp41-5多肽mAb,并用ELISA法对其特异性、抗原识别表位及相对亲和力等做了初步鉴定。获得了4株抗gp41-5多肽的mAb,这4株mAb均特异识别gp41-5多肽,但不与gp41的N36或C34多肽片段反应。得到的4株mAb能特异结合gp41核心结构的空间构象。
To prepare monoclonal antibodies （mAbs） against peptide gp41-5 for analyzing epitopes of gp41 and screening peptides competing with gp41, the mAbs against gp41-5 were made by routine procedures： immunizing mice, fusing cell and cloning. Then specifities, epitopes recognization and relative affinities of mAbs were identified by ELISA. Four strains mAbs were obtained. All of them could recognize gp41-5 specifically, but could not reactive with peptide N36 or C34.The results suggested the four antibodies can specifically bind to the core space structure of gp41.
出处
《科学技术与工程》
2006年第2期127-130,共4页
Science Technology and Engineering
