摘要
研究用于癌症诊断与治疗的光敏剂血卟啉(hematoporphyrin derivative,HPD)的超快光动力学过程。采用超短脉冲激光光谱技术和皮秒时间相关单光子计数系统,测量经血卟啉培养的活体癌细胞与正常细胞的荧光光谱、荧光寿命特性及荧光峰值强度随时间的变化,观测到:癌细胞样品在645nm处具有特征发射光谱峰;癌细胞与正常细胞样品荧光寿命的快成分分别为150,300ps;癌细胞与正常细胞的荧光峰值强度经12h分别衰减10%和55%。对测量所得的荧光光谱曲线及时间分辨荧光衰减曲线分析,计算出:在癌细胞内部血卟啉浓度增大了约2个数量级;癌细胞与正常细胞的荧光寿命分别为824,1798ps;血卟啉在癌细胞与正常细胞样品中滞留时间分别为17,6d。测量结果确认了荧光光谱技术诊断与治疗癌症的可行性,并对发展超短脉冲激光光谱技术早期诊断与治疗癌症具有重要的指导意义和临床应用价值。
In biomedicine research field, the fluorescent phenomena of biological sample is feeble and its time relaxation mostly is between fs and ns. By means of ultrashort pulses laser, feeble fluorescence of the biological sample can be detected expediently and efficiently. The object of this study was to explore the ultrafast photodynamic process of hematoporphyrin derivative （HPD） which is a kind of photosensitisers for diagnosing and therapying cancer. By means of ultrafast pulses laser spectral technique and picosecond time-correlated singlephoton counting system, fluorescence spectrum, the characteristic of fluorescence lifetime and fluorescence peak intensity fluctuation dependent on time of cancer cells sample and normal cells sample were measured. It was observed that cancer ceils sample had special spectral peak at near 645 nm. Moreover, fluorescence lifetime＇s fast part of cancer cells and normal cells were about 150 and 300 ps respectively, and fluorescence intensity of cancer cells and normal cells decayed approximate 10% and 55% separately in twelve hours.According to the measured fluorescence spectrum curve and analyzed time-resolved fluorescence decay curve, it was calculated that the HPD concentration in cancer cells sample increased two orders of magnitude while the HPD concentration in normal cells sample had no apparent change. What＇s more, it was computed that the fluorescence lifetimes of cancer cells and normal cells were about 824 and 1 798 ps and that HPD＇s stay time in cancer cells sample and normal ceils sample were about that the differences between cancer cells and normal fluorescence lifetime and the fluorescence peak intensity 17 days and 6 days respectively. The results indicated cells in fluorescence spectrum, the characteristic of dependent on time were large. This study demonstrated that it was feasible to diagnose and therapy cancer using fluorescence spectrum technique. The study also provides an important guidance for further studying and developing the technique of diagnosing and therapying early cancer using ultrashort pulses laser technique and is significant to clinic application.
出处
《发光学报》
EI
CAS
CSCD
北大核心
2006年第1期113-117,共5页
Chinese Journal of Luminescence
基金
浙江省自然科学基金资助项目(M603265)
关键词
荧光光谱测量
超短脉冲激光
时间相关单光子计数
癌细胞
fluorescence spectrum
ultrashort pulses laser
time-correlated
single-photon counting
cancer cells
