摘要
目的研究大鼠在体与离体动物模型肺泡液体清除率。方法将Evans蓝(0.15 mg/mL)标记5%白蛋白等渗生理盐水溶液及特布他林、氨氯吡咪混合后分别灌注到在体与离体大鼠肺内,根据灌注前及孵育后白蛋白浓度的变化计算肺泡液体清除率。结果在体和离体大鼠肺泡液体清除率在15 min内差异无显著性,30 min离体大鼠肺泡液体清除率是在体大鼠的83%,1 h离体大鼠肺泡液体清除率仅为在体大鼠的60%。氨氯吡咪分别使在体和离体肺脏肺泡液体清除率降低了54.3%和50.4%,特布他林分别使在体和离体肺脏肺泡液体清除率增加了49.7%和44.5%。结论虽然离体肺模型肺泡液体清除率低于在体肺模型,但两者对于药物的反应都是相似的。肺脏离体短时间内,可用于肺泡液体清除功能的研究。
Objective To compare the efficacy of alveolar fluid clearance （AFC） in the isolated lung and live ventilated lung models in rats, Methods Isotonic 5 % Evans blue-labeled albumin solutions with different pharmacotherapeutic agents were instilled into the distal airways of the lungs of both models. AFC was estimated by Evans blue-labeled albumin at progressively increasing concentrations. Results During the first 15 min, AFC was equal in the isolated lungs and live ventilated lungs in rats. However, At 30 min after treatment, AFC in the isolated lungs was decreased to 86% of the live ventilated lungs in rats, At 60 rain after treatment, AFC in the isolated lungs was 60% of the live ventilated lungs. When 10-4 mol/L amiloride was added into the instillation solution, a 54.3 % inhibition of alveolar fluid clearance in the live ventilated lungs in rats and 50.4 % inhibition in the isolated rat lungs were induced. Terbutaline increased alveolar fluid clearance 49.7 % in the live ventilated lungs in rats and 44.5 % in the isolated rat lungs. Conclusions Although the basal AFC in the isolated lung model is slower than that of live ventilated lung models, the effect of drug on AFC in the live ventilated and isolated rat lung models is similar. AFC can be quantified in an isolated rat lung model, it is feasible to use live ventilated or isolated model for investigations of alveolar epithelial fluid transport. The simpler isolated lung model should be useful for short-term studies of alveolar fluid clearance.
出处
《中国比较医学杂志》
CAS
2006年第1期5-8,共4页
Chinese Journal of Comparative Medicine
关键词
模型
动物
肺泡液体清除率
肺水肿
Models, Animal
Alveolar fluid clearance
Pulmonary edema
Rat
