摘要
为观察人参皂苷Rg_2对缺氧大鼠海马神经元的保护作用,选取Wistar大鼠海马神经元体外培养14d,随机分为对照组、5μmol/L尼莫地平组(尼莫地平组)、Rg_20.025mmol/L组(Rg_21组)、Rg_20.050mmol/L组(Rg_22组)。将相应药物加入到培养液中孵育4h后,建立急性缺氧细胞模型。采用流式细胞仪检测各组神经元缺氧后的细胞凋亡情况,用酶法测定超氧化物歧化酶(SOD)的活性以及丙二醛(MDA)、一氧化氮(NO)的含量,并对所得结果加以比较。结果发现,离体条件下人参皂苷Rg_2可显著增强海马神经元的活性,降低其凋亡率及死亡率;提高细胞上清液中超氧化物歧化酶的活性,降低MDA及NO的含量,且呈量效比例关系。以上结果提示,人参皂苷Rg_2在体外有抗缺氧损伤的作用。其作用机制可能是通过抑制细胞凋亡、提高抗氧化酶的活力、清除自由基而发挥作用。
To investigate the neuroprotective effect and its mechanism of Rg2 on anoxic hippocampal neurons of rats, the hippocampal neurons of Wistar rats were cuhured for 14 days. They were randomly divided into four groups： control group, nimodipine group（ 5 μmol/L）, Rg2 1 （0. 025 mmol/L） and Rg2 2 （0.05 mmol/L） group. All neurons of each group were made into acute anoxic models after corresponding drugs were put into culture medium for 4 hours. The apoptosis rate and death rate of hippocampal neurons were tesed by flow cytometry （ FCM）, the contents of MDA and NO and the activities of SOD in the supernatants of ceils were examined by immunochemical technique. The resuhs showed that ： In vitro, Rg2 significantly increased the activities of hippocampal neurons, decreased the apoptosis rate and the death rate, increased the activities of SOD and decreased the contents of MDA and NO in the supernatants of cells. The above results suggest that the Rg2 can protect hippocampal neurons against anoxic injury. Its mechanism may be that the Rg2 can inhibit the apoptosis, eliminate the free radicals and increase the activities of antioxidative enzymes.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2006年第1期99-102,共4页
Chinese Journal of Neuroanatomy
