摘要
目的研究CC型趋化因子家族中调节激活正常T细胞表达和分泌的细胞因子(RANTES)、单核细胞趋化蛋白-1(MCP-1)、单核细胞趋化蛋白-3(MCP-3)和巨噬细胞炎症蛋白(MIP-1α)的mRNA在慢性荨麻疹患者外周血单一核细胞中的表达情况及其相互关系;探讨CC型趋化因子的水平与慢性荨麻疹患者血清总IgE和嗜酸粒细胞阳离子蛋白水平的相关性。方法逆转录聚合酶链反应(RT-PCR)方法检测31例慢性荨麻疹患者和22例正常人对照外周血单一核细胞中RANTES、MCP-1、MCP-3和MIP-1α的mRNA的表达水平。结果①与正常人对照相比,慢性荨麻疹患者外周血单一核细胞中RANTES、MCP-1、MCP-3和MIP-1α的mRNA表达水平均明显增高(P<0.001)。②慢性荨麻疹患者中RANTESmRNA的表达水平与MCP-1、MCP-3、MIP-1α的水平呈正相关(P<0.05),MCP-1与MIP-1α的mRNA表达水平之间亦呈正相关(P<0.01),但MCP-1与MCP-3之间、MCP-3与MIP-1α之间的mRNA表达水平无显著相关(P>0.05)。③慢性荨麻疹患者中RANTES的mRNA表达水平与血清总IgE和嗜酸粒细胞阳离子蛋白的水平呈负相关(P<0.05);MCP-1、MCP-3和MIP-1α的mRNA表达水平与血清总IgE和嗜酸粒细胞阳离子蛋白的水平之间无显著相关(P>0.05)。结论慢性荨麻疹患者外周血单一核细胞中RANTES、MCP-1、MCP-3和MIP-1α的mRNA表达水平的上调可能与荨麻疹的发病有关。
Objective To investigate the mRNA expression of CC Chemokines, including RANTES （regulated upon activation, normal T cell expressed and secreted ）, monocyte chemoattracctant protein-1 （ MCP-1 ）, monocyte chemoattracctant protein-3 （ MCP-3 ） and macrophage inhibitory protein 1α （ MIP-1α ）, in peripheral blood mononuclear cells （ PBMCs ） of patients with chronic urticaria, their mutual correlation, and the relationship between the expression level of CC chemokines and the serum level of IgE and eosinophil cationic protein （ ECP ） in the patients. Method Reverse transcription-polymerase reaction （RT-PCR） was applied to semiquantitatively analyze the mRNA expression of RANTES, MCP-1, MCP-3 and MIP-1α in PBMCs from 31 patients with chronic urticaria and 22 healthy subjects. Results The mRNA expression of RANTES, MCP-1, MCP-3 and MIP-1α was significantly higher in the patients than those in the normal controls （ P 〈 0.001 ）. There was a positive correlation （ P 〈 0.05 ） between the mRNA expression of RANTES and that of MCP-1, MCP-3 and MIP-1α in the patients; the mRNA expression of MCP-1 positively correlated （ P 〈 0.01 ） with that of MIP-1α, while there was no correlation between the mRNA expression of MCP-1 and MCP-3, or between that of MCP-3 and MIP-1α（p 〉 0.05 ）. In the patients, the mRNA expression of RANTES negatively correlated with the serum level of IgE and ECP （ P 〈 0.05 ）. Conclusion The results suggest that the up-regulation of the mRNA expression of RANTES, MCP-1, MCP-3 and MIP-1α might play a role in the pathogenesis of chronic urticaria.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2006年第2期74-76,共3页
Chinese Journal of Dermatology
关键词
荨麻疹
趋化因子
CC
白细胞
单核
Urticaria
Chemokines, CC
Leukocytes, mononuclear
