摘要
目的:改良毛乳头细胞(DPC)分离方法,确立最佳DPC培养条件,界定保持DPC特性的最大传代。方法:使用改良DPC分离法分离纯化人头皮DPC,以不同条件培养,从生长曲线和凋亡两个角度,观察DPC体外生长变化。结果:改良的分离法操作更灵活,DPC获得量充足,污染几率下降;DPC在高糖DMEM+12%FBS环境内生长活力最强,传至6代以后失去原有特性,不再适合DPC体外功能的研究。结论:改良DPC分离法及稳定培养体系的建立,为DPC功能研究奠定了良好的基础。
Objective： To modify the method of dermal papilla cells （DPC） isolation, establish the optimal culture condition of DPC and determine the highest passages in which DPC still sustained their properties. Methods： DPC were dissected from human scalps according to the modified method of DPC isolation established by us, and then cultured in different culture mediums. The DPC growth character was otzserved using MTY and caspase - 3/7 determination. Results： Modified DPC isolation method was more dexterous, the quantity of DPC isolated from specimen was more sufficient, and the contamination probability was descent markedly. DPC grew more actively in high glucose DMEM with 12%FBS. When subculture pasted 5 or 6 passages, the cells lost their properties and no longer fit to functional research in vitro. Conclusion： The establishment of modified DPC isolation and stable culture system laid a solid foundation for DPC functional research.
出处
《中国麻风皮肤病杂志》
2006年第1期28-31,共4页
China Journal of Leprosy and Skin Diseases
基金
国家自然科学基金项目(编号:30200249)
关键词
毛乳头细胞
细胞分离
细胞培养
dermal papilla cells
cell isolation
cell culture
