摘要
目的:观察大豆磷脂酰胆碱对幼年大鼠胸段脊髓前角内突触数量和超微结构的影响,探讨大鼠脊髓神经突触可塑性及大豆磷脂酰胆碱作用机制。方法:①实验于2004-03/12在兰州大学基础医学院神经研究室完成。选用5周龄健康雄性Wistar大鼠22只。随机将大鼠分为2组:大豆磷脂酰胆碱添加喂养组和对照组,每组11只。大豆磷脂酰胆碱添加喂养组:大豆磷脂酰胆碱500mg/(kg·d),溶于50mL双蒸水灌胃,1次/d;对照组:等量双蒸水灌胃,1次/d。两组均自由进食(标准基础饲料)和普通饮水。②喂养8周,灌注取材,用透射电镜观察两组大鼠胸段脊髓灰质前角内突触数密度和突触活性区膜面积密度的变化。用点阵测试系统和点计数法测算脊髓胸段前角灰质内突触的数密度和突触活性区的膜面积密度。③计量资料差异比较采用t检验。结果:大鼠22只均进入结果分析。大豆磷脂酰胆碱添加喂养组大鼠胸段脊髓前角灰质内突触的数密度和突触活性区的膜面积密度均明显高于对照组[(38.27±3.98)个/μm3,(0.0587±0.007)μm2/μm3;(18.36±3.14)个/μm3,(0.0264±0.009)μm2/μm3,t=13.032,9.181,P<0.01]。结论:添加大豆磷脂酰胆碱喂养幼年大鼠,大鼠脊髓突触数量和突触活性区膜面积增加,提高了幼年大鼠脊髓神经突触可塑性。
AIM：To observe the effects of phosphatidylcholine from soybean lecithin （SB-pc） on the synaptic quality and uhrastructure in the comu anterius medullae spin＇dis of thoracic segments in young rats, and investigate the spinal nervous synaptic plasticity as well as the mechanism of spinal synaptic plasticity and the effective action mechanism of SB-pc.
METHODS： ① The experiment was conducted in the Laboratory of Nevous Reseach, Basic Medical College of Lanzhou University from March to December 2004. A total of 22 healthy male Wistar rats of 5 weeks old were randomly divided into two groups： SB-pc additive feeding group and control group with 11 rats in each group. Rats in the SB-pc additive feeding group received gastric perfusion of lecithin （500 mg/kg/d） dissolved in 50 ml distilled water once a day, Rats in the normal control group received gastric perfusion of 50 ml distilled water once a day. Rats in both groups ate （standard basic feeder） and drank freely during the experimental process.②After fed for 8 weeks, materials were drew by perfusion, changes of synaptic numerical density of anterior gray horn in pars thoracica medullae spinalis as well as the decsity of media area in synaptic active area of rats in both groups were observed under transmission electron microscope. The synaptic numerical density of anterior gray horn in pars thoracica medullae spinalis as well as the decsity of media area in synaptic active area were measured with lattice test system and point counting method.③ Differences of measurement data were compared with t test.
RESULTS： A total of 22 rats were involved in the analysis of results. The synaptic numerical density of anterior gray horn in pars thoracica medullae spinalis and the deesity of media area in synaptic active area in the SB-pc additive feeding group were higher than those in the control group [（38.27±3.98）/μm^3, （0.058 7±0.007）μm^2/μm^3; （18.36±3.14） /μm^3, （0.026 4 ±0.009） μm^2/μm^3,t=13.032,9.181, P 〈 0.01].
CONCLUSION： The synaptic number in pars thoracica medullae spinalis and the media area in synaptic active area of rats fed with additive SB-pc are increased, and the synaptic plasticity of spinal nerves in young rats are enhanced.
出处
《中国临床康复》
CSCD
北大核心
2006年第4期107-109,共3页
Chinese Journal of Clinical Rehabilitation
