摘要
背景与目的:血清甘氨酰脯氨酸二肽氨基肽酶同工酶快带(fastbandofglycylprolinedipeptidylaminopeptidaseisoenzyme,GPDA-F)有助于肝癌尤其是甲胎蛋白阴性肝癌的诊断。聚丙烯酰胺凝胶电泳法分离检测GPDA-F方法繁琐,限制了临床应用。本研究旨在建立简便易行的免疫电泳检测法测定GPDA-F,并探讨GPDA-F对肝细胞癌的诊断价值。方法:纯化血清GPDA-F制备多克隆抗体,建立GPDA-F免疫电泳检测法。采用免疫电泳法和聚丙烯酰胺凝胶电泳法同步检测99例肝癌和115例良性肝病患者(其中36例肝硬化、23例急性肝炎、38例慢性肝炎、18例良性肝占位)血清GPDA-F,比较分析免疫电泳法检测血清GPDA-F对肝癌的诊断价值。结果:免疫电泳法定量检测血清GPDA-F结果显示,以71u/L为鉴别界值,其对肝癌的诊断敏感性、特异性和准确性分别为83.8%、85.2%和84.6%,聚丙烯酰胺凝胶电泳法对肝癌的诊断敏感性、特异性和准确性分别为81.8%、77.3%和79.4%。结论:免疫电泳法检测GPDA-F对肝癌有较高的诊断价值,与聚丙烯酰胺凝胶电泳法相比本法有成本低、省时、操作简便等优点。
BACKGROUND ＆ OBJECTIVE： Serum fast band of glycylproline dipeptidyl aminopeptidase isoenzyme （GPDA-F） is useful to the diagnosis of hepatocellular carcinoma, especially for the cases without expression of alpha-fetoprotein （AFP）. Polyacrylamide electrophoresis for detection of GPDA-F is relatively complicated and has limitation in its clinical use. This study was to establish a simple and easy method of immunoelectrophoresis to detect serum GPDA-F, and evaluate clinical value of GPDA-F in the diagnosis of hepatocelluar carcinoma. METHODS： Serum GPDA-F was purified to raise polyclonal GPDA-F antibody, and immunoelectrophoresis was established for the detection of serum GPDA-F. Serum GPDA-F in 99 specimens of hepatocellular carcinoma and 115 specimens of benign liver diseases （36 cases of liver cirrhosis, 23 cases of acute hepatitis, 38 cases of chronic hepatitis, and 18 cases of benign liver space-occupying lesions） was simultaneously detected by both polyacrylamide electrophoresis and immunoelectrophoresis. The clinical value of serum GPDA-F detected by immunoelectrophoresis was compared with that by polyacrylamide electrophoresis for the diagnosis of hepatocellular carcinoma. RESULTS：If the cut-off was set at 71 u/L, the diagnostic sensitivity, specificity, and accuracy of immunoelectrophoresis in detecting GPDA-F in hepatocellular carcinoma were 83.8%, 85.2%, and 84.6%, respectively; while those of polyacrylamide electrophoresis were 81.8% , 77.3%, and 79.4%, respectively. CONCLUSIONS： Serum GPDA-F detected by immunoelectrophoresis is useful to the diagnosis of hepatocellular carcinoma. Compared with polyacrylamide electrophoresis, this method is cheap, time-saving, and easy.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2006年第2期247-249,共3页
Chinese Journal of Cancer
基金
江苏省委"333"工程
南通市社会发展科技项目基金(No.S2014)~~
关键词
肝肿瘤
甘氨酰脯氨酸二肽氨基肽酶
免疫电泳
诊断
Liver neoplasms
Glycylproline dipeptidyl aminopeptidase
Immunoelectrophoresis
Diagnosis
