摘要
目的探讨白细胞介素1B（interleukin1B，IL1B）基因启动子区-511位点C／T基因多态性与冠心病（coronary heart disease，CHD）严重程度的相关性。方法采用聚合酶链反应．限制性片段长度多态性分析的方法，检测127例CHD患者和152名对照组的IL1B-511位点C／T基因型；采用酶法测定血脂各项水平。结果IL1B-511位点C／T基因多态性在急性冠脉综合征（acute coronary syndrome，ACS）组和对照组间的分布差异存在统计学意义（x^2=5．72，P〈0．01），CT及TT基因型患者患ACS的相对风险度约是CC基因型的2．56倍（比值比=2．56，95％可信区间=1．17—5．59）；CHD组中，携带T等位基因的患者血清总胆固醇（6．09±0．97）mmol／L及低密度脂蛋白．胆固醇（3．97±0．92）mmol／L水平显著高于其他患者（5．12±0．56）mmol／L及（2．87±0．71）mmol／L（P〈0．05）。结论IL1B-511位点C／T基因多态性与CHD的严重程度存在相关，其机理可能是该位点DNA变异影响了IL1B的分泌加重了炎症的反应及血脂紊乱。
Objective To investigate the correlation between polymorphism at position - 511C/T in the promoter region of interleukin 1B（IL1B ） and the severity of coronary heart disease （CHD）. Methods The polymerase chain reaction-restriction fragment length polymorphism technique was applied to analyze the polymorphisms of IL1B - 511C/T in 127 patients with CHD and 152 controls. And the serum level of lipoproteins was detected by enzymology method. Results The distribution of IL1B - 511C/T polymorphism between acute coronary syndrome （ACS） patients and controls was significantly different（x^2 = 5.72, P 〈 0.01）. CT and TF genotype carriers were in increased risk of ACS with more double ratio to CC genotype （OR= 2.56, 95%CI = 1.17-5.59）. In CHD group, total cholesterol and low density lipoprotein-cholesteml levels of patients with CT and TT genotypes（6.09±0.97 mmol/L and 3.97±0.92 mmoVL） were significantly higher than those of patients with CC genotype（5.12 ±0.56 mmol/L and 2.87±0.71 mmol/L, P 〈 0.05）. Conclusion The polymorphism at position - 511C/T in IL1B is associated with the severity of CHD, and the DNA variation at this position may affect the secretion of IL1B, and aggravate the reaction of inflammation and dyslipoidemia.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2006年第1期86-88,共3页
Chinese Journal of Medical Genetics
基金
新疆维吾尔自治区自然科学基金(200321105)~~
