摘要
介绍了RNA干扰(RNAi)的概念、线虫中RNAi的作用机理、RNAi的三个分子特性,即可遗传性、转移性RNAi的放大效应和RNAi信号的传播特性。从模板dsRNA的获取、dsRNA的导入和线虫中RNAi效应的检测方法三个方面概述了应用RNAi技术研究线虫基因功能的试验方法和最新进展。
Concept, mechanism, heritability, transitivity and spreading characteristics of RNA interference in parasitic nematode were introduced. Experimental methods for RNAi in parasitic nematode, including acquirement of dsRNA, introduction of dsRNA and detection of RNAi effect in nematode, and new developments in studies of gene functions of parasitic nematode were also reviewed.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2006年第1期80-84,共5页
Chinese Veterinary Science
基金
国家杰出青年科学基金项目(30225033)
广东省自然科学基金重点项目(36835)
广东省科技计划项目(2004B50201020)
关键词
RNA干扰
线虫
基因功能
RNA interference
parasitic nematode
gene function
