摘要
为探讨日粮核苷酸对小鼠炎症/抗炎症平衡体系的作用,用CpG DNA和脂多糖(LPS)刺激小鼠构建炎症反应模型,观察分别饲喂无核苷酸(NF)日粮和核苷酸(NT)日粮小鼠的炎性细胞因子IL-1、IFN-γ、抗炎性细胞因子IL-10水平和小肠髓过氧化物酶(MPO)活性变化。结果显示,在刺激后第4 h时,NT组小鼠的IL-1(P<0.05)、IFN-γ(P<0.01)和小肠MPO(P<0.01)明显低于NF组,IL-10(P>0.05)高于NF组;刺激后第18 h时,NT与NF组的IL-1(P<0.01)、IFN-γ(P<0.01)I、L-10(P>0.05)和MPO(P<0.01)均较刺激后第4 h有所降低,NT组的IL-1(P>0.05)、IFN-γ(P<0.05)和小肠MPO(P<0.05)仍低于NF组,IL-10(P>0.05)仍高于NF组。表明,日粮核苷酸对CpG DNA或LPS所诱导的炎症反应有明显的抑制作用,有助于维持机体炎症/抗炎症平衡,保护机体免受炎症损伤。
The aim of this study was to evaluate effect of dietary nucleotides on inflammation and antiinflammation homeostasis. Mice were administrated with nucleotide-free（NF） or nucleotide-supplemented （NT） diet, and inflammatory cytokine IL-1,IFN-λ, anti-inflammatory cytokine IL-10 and intestinal myeloperoxidase（MPO） were determined at 4th and 18th h after stimulation with CpG DNA and LPS. Production of IL-1（P〈0.05） ,IFN-λ（P〈0.01） and activity of MPO（P〈0.01） decreased, whereas production of IL-10（P〉0.05）increased with nucleotides supplement at 4th h after stimulation. At 18th h after stimulation, production of IL-1（P〈0.01）, IFN-λ（P〈0.01）, IL-10（P〉0.05） and activity of MPO（P〈0.01）were lower than that at 4th h. Production of IL-1（P〈0.05）,IFN-λ,（P〈0.01） and activity of MPO （P〈0.01） in Groups NT were still lower than that in Groups NF, and production of IL-10（P〉0.05） was still higher than that in Groups NF. The results indicated dietary nucleotides can depress inflammation induced by CpG DNA or LPS, maintain inflammatory and anti-inflammatory balance and protect animals from injury.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2006年第1期66-69,共4页
Chinese Veterinary Science
基金
教育部高校骨干教师资助项目(G0202)
