摘要
目的探讨c-met、E-钙黏蛋白(E-cadherin)、β-连环蛋白(-βcatenin)在肝细胞癌(HCC)侵袭转移中的作用,旨在初步明确其在HCC预后判断中的作用。方法采用免疫组化(ABC-HRP)对47例肝细胞癌手术切除标本中c-met、E-cadherin、-βcatenin的表达进行了检测,评估c-met的表达与HCC临床病理因素的关系及与E-cadherin、-βcatenin表达的关系。结果c-met、E-cadherin、-βcatenin在肝细胞癌组织中阳性表达率分别为38.3%、59.6%、38.3%,在正常肝组织中分别为12%、84%、64%,两者比较有显著性差异(P<0.05)。c-met阳性表达率在肝细胞癌的转移(P<0.05)、淋巴浸润(P<0.05)、包膜形成(P<0.05)以及细胞分化方面有显著性差异(P<0.05);而在性别、年龄、肿瘤的大小、分期方面则没有差异。E-cadherin、-βcatenin的表达在肝细胞癌的转移(分别为P=0.032,P=0.022)、包膜形成(分别为P=0.016,P=0.034)方面的有显著性差异;c-met表达与E-cadherin、-βcatenin的异常表达比较有显著性差异(分别为P=0.023,P=0.016)。结论上述结果提示在HCC的转移中,c-met可能是通过HGF/c-met通路影响E-cadherin、-βcatenin的改变来促进细胞的扩散进而出现转移,对于HCC的转移、复发及预后判断具有意义。
Objective To investigate the effect of c-met, E-cadherin, β-catenin on the invasion, metastasis and prognosis of hepatocellular carcinoma （HCC）. Methods Immunohistochemistry technique was used to evaluate the expression of c-met, E-cadherin, and β-catenin, and the relationship between their expression and the clinicopathological prognostic parameters was analyzed in 47 HCC. Results The results showed that the positive expression rate of c-met, E-cadherin, β-catenin in HCC tissue （ n=47） was 38.3%, 59.6% and 38.3%, respectively, but in normal liver tissue was 12%, 84% and 64%, respectively. The expression of c-met was significantly higher in HCC than in normal liver tissue, while the expression of E-cadherin and β-catenin were significantly lower in HCC than in normal liver tissue. The positive rate of c met was correlated with lymph node invasion, capsule, metastasis （intra- or extra-hepatic metastasis） and pathological stage, but not with gender, age, tumor size, and stage of the disease. Similarly, the expression of E-cadherin and β-catenin was correlated with capsule, metastasis （intra- or extra-hepatic metastasis）, but not with gender, age, tumor size, lymph node invasion, stage of the disease and pathological stage. A significant correlation was found between c met and abnormal E-cadherin/β-catenin expression （ P=0. 023, P=0. 016 respectively）. Conclusions These results suggest that c met may be involved in the downregulation of E-cadhcrin and β-catenin, via activation of HGF/c met signal pathway. Thus, HGF/c-met pathway may play im portant roles in the invasion, metastasis and prognosis of HCC.
出处
《消化外科》
CSCD
2006年第1期62-66,共5页
Journal of Digestive Surgery
