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Nogo-A在维甲酸诱导SH-SY5Y细胞分化后的表达分布

Expression and distribution of Nogo-A after SH-SY5Y cell differentiation induced with retinoic acid
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摘要 目的研究Nogo-A在维甲酸(RA)诱导SH-SY5Y细胞分化后的表达及分布。方法采用免疫荧光单标记法和免疫荧光双标记法染色,显微镜观察。结果SH-SY5Y细胞经RA诱导后,细胞生长出较长的突起,具有典型的神经元形态。Nogo-A在未分化的SH-SY5Y细胞中主要分布于胞浆中。经RA诱导后,Nogo-A分布于胞浆及突起中,尤以突起末梢生长锥处免疫阳性强度高。结论Nogo-A在RA诱导SH-SY5Y细胞分化后的轴丘和生长锥处的表达提示其在神经元突起生长过程中可能起到重要作用。 Objective To study the expression and distribution of Nogo-A after SH-SY5Y cell differentiation induced with retinoic acid（RA）. Methods Single and double staining immunofluorescence techniques were used. Results SH-SY5Y cells under the induction of RA outgrew neurites, appearing the typical neuronal phenotype. Nogo-A was mainly located in the cytoplasm of SH-SY5Y cells. After the induction ofRA, Nogo-A was located in the cytoplasm and neurites of SH-SY5Y cells, and immuno-positive expression was especially high in growth cones at the end of neurites. Conclusion The expression of Nogo-A in axon hillock and growth cones of SH-SY5Y cells induced with RA indicates that it possibly plays an important role in the process of axonal growth.
作者 赵宇 游思维 刘惠玲 宋朝君 金伯泉 鞠躬 程希平
机构地区 第四军医大学基础部全军神经科学研究所 第四军医大学基础部免疫学教研室
出处 《中华神经医学杂志》 CAS CSCD 2006年第1期2-5,共4页 Chinese Journal of Neuromedicine
基金 国家自然科学基金(30100195)
关键词 NOGO-A SH-SY5Y细胞 维甲酸 分化 Nogo-A SH-SY5Y cells Retinoic acid Differentiation
分类号 R73-3 [医药卫生—肿瘤]
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参考文献19

  • 1Adachi Y,Itoh F,Yamamoto H,et al.Retinoic acids reduce matrilysin (matrix metalloproteinase 7) and inhibit tumor cell invasion in human colon cancer [J].Tumor Biol,2001,22 (4):247-253.
  • 2Klaassen I,Brakenhoff RH,Smeets S J,et al.Expression of retinoic acid receptor gamma correlates with retinoic acid sensitivity and metabolism in head and neck squamous cell carcinoma cell lines [J].Int J Cancer,2001,92(5):661-665.
  • 3Akie K,Dosaka-Akita H,Murakami A,et al.A combination treatment of c-myc antisense DNA with all-trans-retinoic acid inhibits cell proliferation by downregulating c-myc expression in small cell lung cancer[J].Antisense Nucleic Acid Drug Dev,2000,10(4):243-249.
  • 4Choi SH,Kang HK,Im EO,et al.Inhibition of cell growth and telomerase activity of breast cancer cells in vitro by retinoic acids[J].Int J Oncol,2000,17(5):971-976.
  • 5Pendino F,Flexor M,Delhommeau F,et al.Retinoids down-regulate telomerase and telomere length in a pathway distinct from leukemia cell differentiation [J].Proc Natl Acad Sci USA,2001,98 (12):6662-6667.
  • 6Hsu SL,Cheng CC,Shi YR,et al.Proteolysis ofintegrin alpha5 and betal subunits involved in retinoic acid-induced apoptosis in human hepatoma Hep3B cells[J].Cancer Lett,2001,167(2):193-204.
  • 7Raghunath M,Patti R,Bannerman P,et al.A novel kinase,AATYK induces and promotes neuronal differentiation in a human neuroblastoma (SH-SY5Y) cell line [J].Brain Res Mol Brain Res,2000,77(2):151-162.
  • 8Seidenfaden R,Hildebrandt H.Retinoic acid-induced changes in polysialyltransferase mRNA expression and NCAM polysialylation in human neuroblastoma cells[J].J Neurobiol,2001,46(1):11-28.
  • 9Brittis PA,Flanagan JG.Nogo domains and a Nogo receptor:implications for axon regeneration[J].Neuron,2001,30(1):11-14.
  • 10Oertle T,Huber C,van der Putten H,et al.Genomic structure and functional characterisation of the promoters of human and mouse nogo/rtn4[J].J Mol Biol,2003,325(2):299-323.

二级参考文献11

  • 1[1]Dumont AS, Dumont RJ, Oskouian R J. Will improved understanding of the pathophysiological mechanisms involved in acute spinal cord injury improve the potential for therapeutic intervention [J]? Curr Opin Neurol, 2002; 15(6):713-720.
  • 2[2]Spillmamm AA, Bandtlow CE, Lottspeich F, Keller F, Schwab ME. Identification and characterization of a bovine neurite growth inhibitor (bNI-220)[J]. J Biol Chem, 1998; 273(30): 19283-19293.
  • 3[3]Chen MS, Huber AB, Haar ME, Frank M, Schnell L, Spillmann AA, Christ F,Schwab ME. Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1[J]. Nature, 2000; 403(6768): 434-439.
  • 4[4]Grandpre T, Nalamura F, Vartanian T, Strittmatter SM. Identification of the Nogo inhibitor of axon regeneration as a Reticulon protein[J]. Nature, 2000; 403(6768): 439-444.
  • 5[5]Grandpre T, Strittmatter S. Nogo: A molecular determinant of axonal growth and regeneration [J]. Neuroscientist, 2001; 7(5): 377-386.
  • 6[6]Bradbury EJ, Moon LDF, Popat RJ, King VR, Bennett GS, Patel PN, Fawcett JW, McMahon SB. Chondroitinase ABC promotes functional recovery after spinal cord injury [J]. Nature, 2002;416(6881): 636-640.
  • 7[7]Fouad K, Dietz V, Schwab ME. Improving axonal growth and functional recovery after experimental spinal cord injury by neutralizingmyelin associated inhibitors [J]. Brain Res Brain Res Rev, 2001; 36(2-3): 204-212 .
  • 8[8]Behar O, Mizuno K, Neumann S, Woolf CJ. Putting the spinal cord together again[J]. Neuron, 2000; 26 (2): 291-293.
  • 9[9]McKerracher L, Winton M. Nogo on the go [J]. Neuron, 2002; 36(3): 345-348.
  • 10许晓光,朱勇,刘雪松,田莹,曹云新,刘莹,户义,金伯泉.抗人CD100单克隆抗体的制备与初步鉴定[J].细胞与分子免疫学杂志,2003,19(1):80-82. 被引量：12

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中华神经医学杂志
2006年 第1期

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