摘要
目的:了解利多卡因、庆大霉素对海水浸泡的肢体关节爆炸伤的炎症反应和创面感染的影响。方法:新西兰白兔48只,制作肢体关节爆炸伤模型。随机分为利多卡因、庆大霉素、明胶海绵包扎海水浸泡1h组(LGGS组,n=12);百多邦、明胶海绵包扎海水浸泡1h组(BGS组,n=12);12层普通纱布包扎海水浸泡1h组(GS组,n=12)及12层普通纱布包扎1h组(G组,n=12)。检测各组实验前后血白细胞(WBC)、血清C反应蛋白(CRP)、白细胞介素-1、6(IL-1、IL-6)及肿瘤坏死因子α(TNF-α)、丙二醛(MDA)及超氧化物歧化酶(SOD)水平。爆炸后创面及1h、3d、7d、1月作细菌培养。结果:与实验前比较,实验后血WBC减少,IL-6、TNF-1值上升差异显著(P<0.05、P<0.01);MDA及SOD值,LGGS组无显著性差异(P>0.05),另3组具有显著性差异(P<0.05、P<0.01),LGGS组与另3组组间具有统计学差异(P<0.01)。创面培养阳性细菌24种,LGGS组3d时2只兔出现3种条件致病菌,无创面感染;BGS组9只兔感染;另2组全部感染。结论:利多卡因、庆大霉素创面外用,能一定程度抑制海水浸泡的肢体关节爆炸伤脂质过氧化反应,有明显的预防创面感染的作用。
AIM： To investigate the effects of lidocain and gentamycin on inflammation reaction and wound infection of explosive wound in limb arthrosis after seawater immersion. METHODS： The explosive wound were made in 48 New Zealand white rabbits. All rabbits were randomly divided into 4 groups： lidocain, gentamycin and glutin sponge bound up and immersed in seawater 1 h group （LGGS group, n = 12） ; bactroban and glutin sponge bound up and immersed in seawater 1 h group （BGS group, h = 12）; 12 layers common gauze bound up and immersed in seawater 1 h group （GS goup, n = 12） and 12 layers common gauze bound up 1 h group （G group, n = 12）. The changes of blood WBC, CRP, IL - 1, IL - 6, TNF - α, MDA and SOD in each group were tested. The bacterial culture was performed in each group after explosion 1 h, 3 d, 7 d and 1 month. RESULTS： Compared with pre - experiment, the WBC was decreased, the IL - 6 and TNF - α values were increased significantly （ P 〈 0.05, P 〈 0.01）. The contents of MDA and SOD activity didn＇t change markedly in the LGGS group （P 〉 0.05）, but the other groups did （P 〈 0.05, P 〈 0.01）. 24 gramposition bacteria were found in wounds. Three kinds of condition pathgens were cultured out in the group LGGS on the 3rd day, but none of rabbits were infected. 9 rabbits were infected in the group BGS. All rabbits in the other groups were infected. CONCLUSION： Lindocain and gentamycin used externally to the wound restrain tissue lipid perosidation to some extent and prevent wound infection evidently in explosive wound after seawater immersion.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第1期112-115,共4页
Chinese Journal of Pathophysiology
关键词
利多卡因
庆大霉素类
爆震伤
海水浸泡
Lidocaine
Gentamicins
Explosion wound
Seawater immersion
