摘要
目的得到具有抗D-二聚体活性的人源抗体。方法应用噬菌体展示技术构建血栓病人的抗体库,展示了血栓病人的抗体,从中筛选出能与血栓主要成分纤维蛋白降解产物D-二聚体有特异结合作用的抗体。将抗体在大肠杆菌XL1-Blue中表达。再利用抗D-二聚体鼠源抗体进行竞争ELISA检验,证实抗体与D-二聚体结合的活性。结果从血栓病人抗体库中得到抗体p13株,并得到可溶表达,在ELISA检测中显示与D-二聚体结合的优势。结论该抗体株p13可供进一步开展导向溶栓剂的研究。
Objective To pick out a human antibody that could interacted with D-dimer. Methods A human phage-displayed Fab library was constructed by using phage surface display technology. The antibodies, which could interact with D-dimer, were obtained by biopanning, and then expressed in XL1-Blue. The D-dimer-binding activity of the antibodies was assured by a competition ELISA test. Results The antibody numbered p13 was solubly expressed in XL1-Blue, and had a higher binding activity to D-dimer proved by ELISA. Conclusion The success of isolating human anti-D-dimer Fab p13 provides a base for further study of targeted thrombolytic.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2006年第1期34-36,39,共4页
Immunological Journal
基金
广州市重点科技攻关资助项目(1999-X-006-01)
