摘要
目的探讨微循环障碍在急性坏死性胰腺炎大鼠中的作用，同时观察川芎嗪对急性坏死性胰腺炎胃粘膜损伤的干预效应。方法SD大鼠192只，随机分对照组（C）、胰腺炎组（P）、川芎嗪治疗组（T）。经大鼠胰腺被膜下均匀注射5％牛磺胆酸钠（4ml·kg^-1）制作急性坏死性胰腺炎模型，C组仅胰腺被膜下注射等量生理盐水，T组经股静脉注射0.6％川芎嗪（10mi·kg^-1）。采用放射性生物微球技术在制模后0．5、2、6及12h分别测定胃的血流量.另8只用于胰腺、胃的病理评分并观察胃组织的MPO活性。结果P组与C组相比，P组各时相胃的血流量均明显降低（p〈0.01）。各时相病理改变明显加重，时间越长，损害越重；胃组织MPO活性明显增加（P〈0．01）。T组与P组相比，T组2h起胃血流量较P组均明显降低（P〈0．01），各时相病理改变及白细胞浸润明显减轻，组织MPO活性明显下降（P〈0．05或P〈0.01）。胃组织血流量与胃组织MPO活性间呈显著负相关，与胃组织损伤程度呈显著负相关。结论微循环障碍在急性坏死性胰腺炎时胃组织损伤中起着重要的作用。川芎嗪可以改善微循环，减轻胰腺及胃粘膜损害。
Objective The purpose of this study was to evaluate the role of microcirculatory disorder（MCD） ,and study the therapeutic ef fectiveness of ligustrazine to the gastric mucosa injury on acute necrotizing pancreatitis in rats.Methods A total of 192 SD rats were random divided into three group： normal control group（C） ;ANP group without treatment（P） ;ANP group treated with ligustrazine（T）. The ANP model was induced by injection of 5 %sodium tauroeholate under the pancreatic membrane（4m·kg^-1）. Normal control group was received isovolumet-ric injections of 0.9% physiological saline solution using the same method. T group was injected of ligustrazine（ 10ml·kg^-1）via portal vein. Radioactive biomicrosphere technique（RMT） was used to measure the blood flow at 0.5h,2h,6h and 12h after the induction of ANP. The samples of pancreas, stomach were collected to observe pathological changes using a validated histology score. Gastric tissues were also used for examination of myeloperoxidase（ MPO）. Results Comparison of P group and C group, the blood flow in P group was significantly lower than that of C group（P 〈 0.01） ; The pathological changes were aggravated significantly in P group. The time longer, the severer. Gastric MPO activity in P group were significantly higher than those in C group（P 〈 0.01 ）. Comparison of T group and P group, the blood flow of intestine in T group Was significantly higher than that of P group after 2 h（P 〈 0.01）. The pathological changes were alleviated significantly in T group. MPO activity were significantly lower than those in Pgroup（P 〈 0.01 or P 〈 0.05）. There were significant negative correlation bet ween the gastric blood flow and M PO activity and the pathological changes of gastric tissues in P group. Conclusion MCD is an important factor of gastric injury in ANP. Ligustrazine can ameliorate the condition of MCD and alleviate the damage of pancreas and stomach.
出处
《现代保健》
2006年第01X期1-4,共4页
Modern Health
关键词
胰腺炎
微循环
川芎嗪
胃粘膜损害
Pancreatitis Microcireulation Ligustrazine Gastric mucosal lesion
