摘要
目的:比较2种处理方法对雷公藤饮片中雷公藤内酯醇含量测定的影响。方法:对雷公藤饮片水煎液浓缩后,分别采取中性氧化铝吸附后柱层析分离(方法1)和经乙醇沉淀后以氯仿萃取再经中性氧化铝柱层析分离(方法2)的方法进行处理,以高效液相色谱法测定雷公藤内酯醇的含量,并对含量测定结果进行配对t检验。结果:方法1所测定的含量明显高于方法2,具有显著性差异(P<0·05)。结论:方法1准确性和灵敏度高,比方法2更适合于雷公藤饮片中雷公藤内酯醇含量的测定。
OBJECTIVE： To compare the effects of 2 different treatment methods on the assaying of triptolide alcohol in tripterygium wilfordii cut crude drug.METHODS： After concentration, tripterygium wilfordii cut crude drug water decoction underwent column chromatographic separation after adsorption with neutral alumina（Method 1） and neutral alumina column chromatographic separation after ethanol precipitation and extraction by chloroform（Method 2）, respectively.Triptolide alcohol was determined by HPLC and the assaying result underwent pairing and t tests.RESULTS： There was significant difference between the 2 methods with the content determined by Method 1 significantly higher than that by Method 2（P 〈 0.05）.CONCLUSION,As compared with Method 2,Method 1 is more accurate and sensitive, and which is more suitable for the assaying of triptolide alcohol in tripterygium wilfordii cut crude drug.
出处
《中国药房》
CAS
CSCD
北大核心
2006年第4期299-300,共2页
China Pharmacy
